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globalchange > 过去全球变化的重建

DOI:	10.1371/journal.pone.0096503
论文题名:	Enhanced Production of Docosahexaenoic Acid in Mammalian Cells
作者:	Guiming Zhu; Xudong Jiang; Qin Ou; Tao Zhang; Mingfu Wang; Guozhi Sun; Zhao Wang; Jie Sun; Tangdong Ge
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2014
发表日期:	2014-5-2
卷:	9, 期:5
语种:	英语
英文关键词:	Fatty acids ; CHO cells ; Hyperexpression techniques ; Green fluorescent protein ; Mammals ; Transfection ; Biosynthesis ; Marine fish
英文摘要:	Docosahexaenoic acid (DHA), one of the important polyunsaturated fatty acids (PUFA) with pharmaceutical and nutraceutical effects, may be obtained through diet or synthesized in vivo from dietary a-linolenic acid (ALA). However, the acumulation of DHA in human body or other mammals relies on the intake of high dose of DHA for a certain period of time, and the bioconversion of dietary ALA to DHA is very limited. Therefore the mammalian cells are not rich in DHA. Here, we report a new technology for increased prodution of DHA in mammalian cells. By using transient transfection method, Siganus canaliculatus Δ4 desaturase was heterologously expressed in chinese hamster ovary (CHO) cells, and simultaneously, mouse Δ6-desaturase and Δ5-desaturase were overexpressed. The results demonstrated that the overexpression of Δ6/Δ5-desaturases significantly enhanced the ability of transfected cells to convert the added ALA to docosapentaenoic acid (DPA) which in turn get converted into DHA directly and efficiently by the heterologously expressed Δ4 desaturase. This technology provides the basis for potential utility of these gene constructs in the creation of transgenic livestock for increased production of DHA/related products to meet the growing demand of this important PUFA.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0096503&type=printable
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资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/18764
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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File Name/ File Size	Content Type	Version	Access	License
journal.pone.0096503.PDF（407KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Laboratory of Biochemistry and Molecular Biology, College of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang, China;Laboratory of Biochemistry and Molecular Biology, College of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang, China;Laboratory of Biochemistry and Molecular Biology, College of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang, China;Laboratory of Biochemistry and Molecular Biology, College of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang, China;Laboratory of Biochemistry and Molecular Biology, College of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang, China;Laboratory of Biochemistry and Molecular Biology, College of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang, China;Laboratory of Biochemistry and Molecular Biology, College of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang, China;Laboratory of Biochemistry and Molecular Biology, College of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang, China;Laboratory of Biochemistry and Molecular Biology, College of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang, China

Recommended Citation:

Guiming Zhu,Xudong Jiang,Qin Ou,et al. Enhanced Production of Docosahexaenoic Acid in Mammalian Cells[J]. PLOS ONE,2014-01-01,9(5)

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