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DOI:	10.1371/journal.pone.0089691
论文题名:	Assessing the Exoproteome of Marine Bacteria, Lesson from a RTX-Toxin Abundantly Secreted by Phaeobacter Strain DSM 17395
作者:	Emie Durighello; Joseph Alexander Christie-Oleza; Jean Armengaud
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2014
发表日期:	2014-2-24
卷:	9, 期:2
语种:	英语
英文关键词:	Peptides ; Chaperone proteins ; Outer membrane proteins ; Serine proteases ; Marine bacteria ; Proteolysis ; Proteomics ; Marine environments
英文摘要:	Bacteria from the Roseobacter clade are abundant in surface marine ecosystems as over 10% of bacterial cells in the open ocean and 20% in coastal waters belong to this group. In order to document how these marine bacteria interact with their environment, we analyzed the exoproteome of Phaeobacter strain DSM 17395. We grew the strain in marine medium, collected the exoproteome and catalogued its content with high-throughput nanoLC-MS/MS shotgun proteomics. The major component represented 60% of the total protein content but was refractory to either classical proteomic identification or proteogenomics. We de novo sequenced this abundant protein with high-resolution tandem mass spectra which turned out being the 53 kDa RTX-toxin ZP_02147451. It comprised a peptidase M10 serralysin domain. We explained its recalcitrance to trypsin proteolysis and proteomic identification by its unusual low number of basic residues. We found this is a conserved trait in RTX-toxins from Roseobacter strains which probably explains their persistence in the harsh conditions around bacteria. Comprehensive analysis of exoproteomes from environmental bacteria should take into account this proteolytic recalcitrance.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089691&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/18902
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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File Name/ File Size	Content Type	Version	Access	License
journal.pone.0089691.PDF（577KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

CEA, DSV, IBEB, Lab Biochim System Perturb, Bagnols-sur-Cèze, France;CEA, DSV, IBEB, Lab Biochim System Perturb, Bagnols-sur-Cèze, France;CEA, DSV, IBEB, Lab Biochim System Perturb, Bagnols-sur-Cèze, France

Recommended Citation:

Emie Durighello,Joseph Alexander Christie-Oleza,Jean Armengaud. Assessing the Exoproteome of Marine Bacteria, Lesson from a RTX-Toxin Abundantly Secreted by Phaeobacter Strain DSM 17395[J]. PLOS ONE,2014-01-01,9(2)

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