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globalchange > 过去全球变化的重建

DOI:	10.1371/journal.pone.0088030
论文题名:	Conditioned Place Avoidance of Zebrafish (Danio rerio) to Three Chemicals Used for Euthanasia and Anaesthesia
作者:	Devina Wong; Marina A. G. von Keyserlingk; Jeffrey G. Richards; Daniel M. Weary
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2014
发表日期:	2014-2-4
卷:	9, 期:2
语种:	英语
英文关键词:	Oils ; Zebrafish ; Fishes ; Anesthesia ; Fish physiology ; Water columns ; Chemical equilibrium ; Conditioned response
英文摘要:	Zebrafish are becoming one of the most used vertebrates in developmental and biomedical research. Fish are commonly killed at the end of an experiment with an overdose of tricaine methanesulfonate (TMS, also known as MS-222), but to date little research has assessed if exposure to this or other agents qualifies as euthanasia (i.e. a “good death”). Alternative agents include metomidate hydrochloride and clove oil. We use a conditioned place avoidance paradigm to compare aversion to TMS, clove oil, and metomidate hydrochloride. Zebrafish (n = 51) were exposed to the different anaesthetics in the initially preferred side of a light/dark box. After exposure to TMS zebrafish spent less time in their previously preferred side; aversion was less pronounced following exposure to metomidate hydrochloride and clove oil. Nine of 17 fish exposed to TMS chose not to re-enter the previously preferred side, versus 2 of 18 and 3 of 16 refusals for metomidate hydrochloride and clove oil, respectively. We conclude that metomidate hydrochloride and clove oil are less aversive than TMS and that these agents be used as humane alternatives to TMS for killing zebrafish.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0088030&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/18925
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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File Name/ File Size	Content Type	Version	Access	License
journal.pone.0088030.PDF（353KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Animal Welfare Program, University of British Columbia, Vancouver, British Columbia, Canada;Animal Welfare Program, University of British Columbia, Vancouver, British Columbia, Canada;Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada;Animal Welfare Program, University of British Columbia, Vancouver, British Columbia, Canada

Recommended Citation:

Devina Wong,Marina A. G. von Keyserlingk,Jeffrey G. Richards,et al. Conditioned Place Avoidance of Zebrafish (Danio rerio) to Three Chemicals Used for Euthanasia and Anaesthesia[J]. PLOS ONE,2014-01-01,9(2)

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