globalchange  > 过去全球变化的重建
DOI: 10.1371/journal.pone.0085119
论文题名:
Membrane Labeling of Coral Gastrodermal Cells by Biotinylation: The Proteomic Identification of Surface Proteins Involving Cnidaria-Dinoflagellate Endosymbiosis
作者: Hsing-Hui Li; Zi-Yu Huang; Shih-Png Ye; Chi-Yu Lu; Pai-Chiao Cheng; Shu-Hwa Chen; Chii-Shiarng Chen
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2014
发表日期: 2014-1-7
卷: 9, 期:1
语种: 英语
英文关键词: Membrane proteins ; Cell membranes ; Chaperone proteins ; Biotinylation ; Corals ; Membrane-associated cytoskeletal proteins ; Phagocytosis ; Sequence databases
英文摘要: The cellular and molecular-scale processes underlying the stability of coral-Symbiodinium endosymbioses remain unclear despite decades of investigation. As the coral gastroderm is the only tissue layer characterized by this unique symbiotic association, the membranes of these symbiotic gastrodermal cells (SGCs) may play important roles in the initiation and maintenance of the endosymbiosis. In order to elucidate the interactions between the endosymbiotic dinoflagellates and their coral hosts, a thorough characterization of SGC membranes is therefore required. Cell surface proteins of isolated SGCs were biotinylated herein by a cell impermeant agent, biotin-XX sulfosuccinimidyl ester. The in situ distribution of these biotinylated proteins was uncovered by both fluorescence and transmission electron microscopic imaging of proteins bound to Alexa Fluor® 488-conjugated streptavidin. The identity of these proteins was then determined by two-dimensional gel electrophoresis followed by liquid chromatography-tandem mass spectrometry. Nineteen (19) proteins were identified, and they are known to be involved in the molecular chaperone/stress response, cytoskeletal remodeling, and energy metabolism. These results not only reveal the molecular characters of the host SGC membrane, but also provide critical insight into understanding the possible role of host membranes in this ecologically important endosymbiotic association.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0085119&type=printable
Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/18982
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

Files in This Item:
File Name/ File Size Content Type Version Access License
journal.pone.0085119.PDF(552KB)期刊论文作者接受稿开放获取View Download

作者单位: Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung, Taiwan;Taiwan Coral Research Center, National Museum of Marine Biology and Aquarium, Pingtung, Taiwan;Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung, Taiwan;Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung, Taiwan;Department of Biochemistry, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan, Taiwan;Institute of Information Science, Academia Sinica, Taipei, Taiwan;Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung, Taiwan;Taiwan Coral Research Center, National Museum of Marine Biology and Aquarium, Pingtung, Taiwan;Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung, Taiwan

Recommended Citation:
Hsing-Hui Li,Zi-Yu Huang,Shih-Png Ye,et al. Membrane Labeling of Coral Gastrodermal Cells by Biotinylation: The Proteomic Identification of Surface Proteins Involving Cnidaria-Dinoflagellate Endosymbiosis[J]. PLOS ONE,2014-01-01,9(1)
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Hsing-Hui Li]'s Articles
[Zi-Yu Huang]'s Articles
[Shih-Png Ye]'s Articles
百度学术
Similar articles in Baidu Scholar
[Hsing-Hui Li]'s Articles
[Zi-Yu Huang]'s Articles
[Shih-Png Ye]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Hsing-Hui Li]‘s Articles
[Zi-Yu Huang]‘s Articles
[Shih-Png Ye]‘s Articles
Related Copyright Policies
Null
收藏/分享
文件名: journal.pone.0085119.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.