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DOI: 10.1371/journal.pone.0084431
论文题名:
Label-Free Imaging of Lipid Depositions in C. elegans Using Third-Harmonic Generation Microscopy
作者: George J. Tserevelakis; Evgenia V. Megalou; George Filippidis; Barbara Petanidou; Costas Fotakis; Nektarios Tavernarakis
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2014
发表日期: 2014-1-2
卷: 9, 期:1
语种: 英语
英文关键词: Lipids ; Caenorhabditis elegans ; Lipid signaling ; Gastrointestinal tract ; Lipid structure ; Acid deposition ; Fats ; Lipid metabolism
英文摘要: Elucidation of the molecular mechanisms regulating lipid storage and metabolism is essential for mitigating excess adiposity and obesity, which has been associated with increased prevalence of severe pathological conditions such as cardiovascular disorders and type II diabetes, worldwide. However, imaging fatty acid distribution and dynamics in vivo, at the cellular or organismal level is challenging. We developed a label-free method for visualizing lipid depositions in vivo, based on third harmonic generation (THG) microscopy. THG imaging requires a single pulsed-laser light source, alleviating the technical challenges of implementing coherent anti-Stokes Raman scattering spectroscopy (CARS) to detect fat stores in living cells. We demonstrate that THG can be used to efficiently and reliably visualize lipid droplets in Caenorhabditis elegans. Thus, THG microscopy offers a versatile alternative to fluorescence and dye-based approaches for lipid biology research.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0084431&type=printable
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/18985
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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作者单位: Institute of Electronic Structure and Laser, Foundation for Research and Technology, Crete, Greece;Physics Department, University of Crete, Crete, Greece;Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Crete, Greece;Institute of Electronic Structure and Laser, Foundation for Research and Technology, Crete, Greece;Institute of Electronic Structure and Laser, Foundation for Research and Technology, Crete, Greece;Physics Department, University of Crete, Crete, Greece;Institute of Electronic Structure and Laser, Foundation for Research and Technology, Crete, Greece;Physics Department, University of Crete, Crete, Greece;Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Crete, Greece;Medical School, University of Crete, Crete, Greece

Recommended Citation:
George J. Tserevelakis,Evgenia V. Megalou,George Filippidis,et al. Label-Free Imaging of Lipid Depositions in C. elegans Using Third-Harmonic Generation Microscopy[J]. PLOS ONE,2014-01-01,9(1)
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