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DOI:	10.1371/journal.pone.0114148
论文题名:	Detection of DNA Sequences Refractory to PCR Amplification Using a Biophysical SERRS Assay (Surface Enhanced Resonant Raman Spectroscopy)
作者:	Cécile Feuillie; Maxime M. Merheb; Benjamin Gillet; Gilles Montagnac; Isabelle Daniel; Catherine Hänni
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2014
发表日期:	2014-12-12
卷:	9, 期:12
语种:	英语
英文关键词:	Polymerase chain reaction ; Sequence alignment ; Ancient DNA ; Probe hybridization ; DNA hybridization ; DNA damage ; Silver ; Oligonucleotides
英文摘要:	The analysis of ancient or processed DNA samples is often a great challenge, because traditional Polymerase Chain Reaction – based amplification is impeded by DNA damage. Blocking lesions such as abasic sites are known to block the bypass of DNA polymerases, thus stopping primer elongation. In the present work, we applied the SERRS-hybridization assay, a fully non-enzymatic method, to the detection of DNA refractory to PCR amplification. This method combines specific hybridization with detection by Surface Enhanced Resonant Raman Scattering (SERRS). It allows the detection of a series of double-stranded DNA molecules containing a varying number of abasic sites on both strands, when PCR failed to detect the most degraded sequences. Our SERRS approach can quickly detect DNA molecules without any need for DNA repair. This assay could be applied as a pre-requisite analysis prior to enzymatic reparation or amplification. A whole new set of samples, both forensic and archaeological, could then deliver information that was not yet available due to a high degree of DNA damage.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0114148&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/18997
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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作者单位:

Laboratoire de Géologie de Lyon – Terre, Planètes, Environnement, UMR 5276, ENS de Lyon, Université Lyon 1, CNRS, Ecole Normale Supérieure de Lyon, 46 allée d′Italie, 69364 Lyon Cedex 07, France;Institut de Génomique Fonctionnelle de Lyon, UMR 5242, Université Lyon 1, CNRS, Ecole Normale Supérieure de Lyon, 46 allée d′Italie, 69364 Lyon Cedex 07, France;Institut de Génomique Fonctionnelle de Lyon, UMR 5242, Université Lyon 1, CNRS, Ecole Normale Supérieure de Lyon, 46 allée d′Italie, 69364 Lyon Cedex 07, France;Plateforme nationale de Paléogénétique PALGENE, CNRS, Ecole Normale Supérieure de Lyon, 46 allée d′Italie, 69364 Lyon Cedex 07, France;Laboratoire de Géologie de Lyon – Terre, Planètes, Environnement, UMR 5276, ENS de Lyon, Université Lyon 1, CNRS, Ecole Normale Supérieure de Lyon, 46 allée d′Italie, 69364 Lyon Cedex 07, France;Laboratoire de Géologie de Lyon – Terre, Planètes, Environnement, UMR 5276, ENS de Lyon, Université Lyon 1, CNRS, Ecole Normale Supérieure de Lyon, 46 allée d′Italie, 69364 Lyon Cedex 07, France;Institut de Génomique Fonctionnelle de Lyon, UMR 5242, Université Lyon 1, CNRS, Ecole Normale Supérieure de Lyon, 46 allée d′Italie, 69364 Lyon Cedex 07, France;Plateforme nationale de Paléogénétique PALGENE, CNRS, Ecole Normale Supérieure de Lyon, 46 allée d′Italie, 69364 Lyon Cedex 07, France

Recommended Citation:

Cécile Feuillie,Maxime M. Merheb,Benjamin Gillet,et al. Detection of DNA Sequences Refractory to PCR Amplification Using a Biophysical SERRS Assay (Surface Enhanced Resonant Raman Spectroscopy)[J]. PLOS ONE,2014-01-01,9(12)

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