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DOI: 10.1371/journal.pone.0110834
论文题名:
Purification and Characterization of a Novel Laccase from Cerrena sp. HYB07 with Dye Decolorizing Ability
作者: Jie Yang; Qi Lin; Tzi Bun Ng; Xiuyun Ye; Juan Lin
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2014
发表日期: 2014-10-30
卷: 9, 期:10
语种: 英语
英文关键词: Laccases ; Sequence motif analysis ; Organic solvents ; Effluent ; Complementary DNA ; Fermentation ; Enzyme assays ; Biodegradation
英文摘要: Laccases (EC 1.10.3.2) are a class of multi-copper oxidases with important industrial values. A basidiomycete strain Cerrena sp. HYB07 with high laccase yield was identified. After cultivation in the shaking flask for 4 days, a maximal activity of 210.8 U mL−1 was attained. A 58.6-kDa laccase (LacA) with 7.2% carbohydrate and a specific activity of 1952.4 U mg−1 was purified. 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) was the optimal substrate, with Km and kcat being 93.4 µM and 2468.0 s−1, respectively. LacA was stable at 60°C, pH 5.0 and above, and in organic solvents. Metal ions Na+, K+, Ca2+, Mg2+, Mn2+, Zn2+ enhanced LacA activity, while Fe2+ and Li+ inhibited LacA activity. LacA decolorized structurally different dyes and a real textile effluent. Its gene and cDNA sequences were obtained. Putative cis-acting transcriptional response elements were identified in the promoter region. The high production yield and activity, robustness and dye decolorizing capacity make LacA and Cerrena sp. HYB07 potentially useful for industrial and environmental applications such as textile finishing and wastewater treatment.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0110834&type=printable
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/19020
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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作者单位: College of Biological Sciences and Technology, Fuzhou University, Fuzhou, Fujian, China;National Engineering Laboratory for Enzyme Expression, Fuzhou, Fujian, China;College of Biological Sciences and Technology, Fuzhou University, Fuzhou, Fujian, China;School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China;College of Biological Sciences and Technology, Fuzhou University, Fuzhou, Fujian, China;National Engineering Laboratory for Enzyme Expression, Fuzhou, Fujian, China;College of Biological Sciences and Technology, Fuzhou University, Fuzhou, Fujian, China;National Engineering Laboratory for Enzyme Expression, Fuzhou, Fujian, China

Recommended Citation:
Jie Yang,Qi Lin,Tzi Bun Ng,et al. Purification and Characterization of a Novel Laccase from Cerrena sp. HYB07 with Dye Decolorizing Ability[J]. PLOS ONE,2014-01-01,9(10)
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