全球变化知识资源中心

Knowledge Resources Center for Global Change


Email:
Passwd
验证:
	换一张
Have you forgotten your password? Stay signed in Log In

globalchange > 过去全球变化的重建

DOI:	10.1371/journal.pone.0101755
论文题名:	Molecular Identification of Adult and Juvenile Linyphiid and Theridiid Spiders in Alpine Glacier Foreland Communities
作者:	Lorna Raso; Daniela Sint; Alexander Rief; Rüdiger Kaufmann; Michael Traugott
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2014
发表日期:	2014-7-22
卷:	9, 期:7
语种:	英语
英文关键词:	Polymerase chain reaction ; Glaciers ; Sequence alignment ; Phylogenetic analysis ; Spiders ; DNA extraction ; Ethanol ; Rickettsia
英文摘要:	In glacier forelands spiders constitute a large proportion of the invertebrate community. Therefore, it is important to be able to determine the species that can be found in these areas. Linyphiid and theridiid spider identification is currently not possible in juvenile specimens using traditional morphological based methods, however, a large proportion of the population in these areas are usually juveniles. Molecular methods permit identification of species at different life stages, making juvenile identification possible. In this study we tested a molecular tool to identify the 10 most common species of Linyphiidae and Theridiidae found in three glacier foreland communities of the Austrian Alps. Two multiplex PCR systems were developed and over 90% of the 753 field-collected spiders were identified successfully. The species targeted were found to be common in all three valleys during the summer of 2010. A comparison between the molecular and morphological data showed that although there was a slight difference in the results, the overall outcome was the same independently of the identification method used. We believe the quick and reliable identification of the spiders via the multiplex PCR assays developed here will aid the study of these families in Alpine habitats.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0101755&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/19072
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

Files in This Item:

File Name/ File Size	Content Type	Version	Access	License
journal.pone.0101755.PDF（939KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Institute of Ecology, University of Innsbruck, Innsbruck, Austria;Institute of Ecology, University of Innsbruck, Innsbruck, Austria;Institute of Ecology, University of Innsbruck, Innsbruck, Austria;Institute of Ecology, University of Innsbruck, Innsbruck, Austria;Institute of Ecology, University of Innsbruck, Innsbruck, Austria

Recommended Citation:

Lorna Raso,Daniela Sint,Alexander Rief,et al. Molecular Identification of Adult and Juvenile Linyphiid and Theridiid Spiders in Alpine Glacier Foreland Communities[J]. PLOS ONE,2014-01-01,9(7)

Service
	Recommend this item
	Sava as my favorate item
	Show this item's statistics
	Export Endnote File
Google Scholar
	Similar articles in Google Scholar
	[Lorna Raso]'s Articles
	[Daniela Sint]'s Articles
	[Alexander Rief]'s Articles
百度学术
	Similar articles in Baidu Scholar
	[Lorna Raso]'s Articles
	[Daniela Sint]'s Articles
	[Alexander Rief]'s Articles
CSDL cross search
	Similar articles in CSDL Cross Search
	[Lorna Raso]‘s Articles
	[Daniela Sint]‘s Articles
	[Alexander Rief]‘s Articles
Related Copyright Policies
Null
收藏/分享