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DOI:	10.1371/journal.pone.0085061
论文题名:	Metabolic Engineering Camelina sativa with Fish Oil-Like Levels of DHA
作者:	James R. Petrie; Pushkar Shrestha; Srinivas Belide; Yoko Kennedy; Geraldine Lester; Qing Liu; Uday K. Divi; Roger J. Mulder; Maged P. Mansour; Peter D. Nichols; Surinder P. Singh
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2014
发表日期:	2014-1-21
卷:	9, 期:1
语种:	英语
英文关键词:	Fatty acids ; Seeds ; Lipids ; Arabidopsis thaliana ; Oils ; Flax ; Genetically modified plants ; Marine fish
英文摘要:	Background Omega-3 long-chain (≥C20) polyunsaturated fatty acids (ω3 LC-PUFA) such as eicosapentaenoic acid (EPA) and docosapentaenoic acid (DHA) are critical for human health and development. Numerous studies have indicated that deficiencies in these fatty acids can increase the risk or severity of cardiovascular, inflammatory and other diseases or disorders. EPA and DHA are predominantly sourced from marine fish although the primary producers are microalgae. Much work has been done to engineer a sustainable land-based source of EPA and DHA to reduce pressure on fish stocks in meeting future demand, with previous studies describing the production of fish oil-like levels of DHA in the model plant species, Arabidopsis thaliana. Principal Findings In this study we describe the production of fish oil-like levels (>12%) of DHA in the oilseed crop species Camelina sativa achieving a high ω3/ω6 ratio. The construct previously transformed in Arabidopsis as well as two modified construct versions designed to increase DHA production were used. DHA was found to be stable to at least the T5 generation and the EPA and DHA were found to be predominantly at the sn-1,3 positions of triacylglycerols. Transgenic and parental lines did not have different germination or seedling establishment rates. Conclusions DHA can be produced at fish oil-like levels in industrially-relevant oilseed crop species using multi-gene construct designs which are stable over multiple generations. This study has implications for the future of sustainable EPA and DHA production from land-based sources.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0085061&type=printable
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资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/19182
Appears in Collections:	过去全球变化的重建科学计划与规划全球变化的国际研究计划影响、适应和脆弱性气候变化与战略气候减缓与适应气候变化事实与影响

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journal.pone.0085061.PDF（592KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

CSIRO Food Futures National Research Flagship, Canberra, Australian Capital Territory, Australia;CSIRO Food Futures National Research Flagship, Canberra, Australian Capital Territory, Australia;CSIRO Food Futures National Research Flagship, Canberra, Australian Capital Territory, Australia;CSIRO Food Futures National Research Flagship, Canberra, Australian Capital Territory, Australia;CSIRO Food Futures National Research Flagship, Canberra, Australian Capital Territory, Australia;CSIRO Food Futures National Research Flagship, Canberra, Australian Capital Territory, Australia;CSIRO Food Futures National Research Flagship, Canberra, Australian Capital Territory, Australia;CSIRO Materials Science and Engineering, Clayton, Victoria, Australia;CSIRO Food Futures National Research Flagship, Hobart, Tasmania, Australia;CSIRO Food Futures National Research Flagship, Hobart, Tasmania, Australia;CSIRO Food Futures National Research Flagship, Canberra, Australian Capital Territory, Australia

Recommended Citation:

James R. Petrie,Pushkar Shrestha,Srinivas Belide,et al. Metabolic Engineering Camelina sativa with Fish Oil-Like Levels of DHA[J]. PLOS ONE,2014-01-01,9(1)

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