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DOI:	10.1371/journal.pone.0092780
论文题名:	Direct Observation of Unstained Biological Specimens in Water by the Frequency Transmission Electric-Field Method Using SEM
作者:	Toshihiko Ogura
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2014
发表日期:	2014-3-20
卷:	9, 期:3
语种:	英语
英文关键词:	Scanning electron microscopy ; Imaging techniques ; Monte Carlo method ; Yeast ; Atmospheric layers ; Radiobiology ; Aluminum ; Square waves
英文摘要:	Scanning electron microscopy (SEM) is a powerful tool for the direct visualization of biological specimens at nanometre-scale resolution. However, images of unstained specimens in water using an atmospheric holder exhibit very poor contrast and heavy radiation damage. Here, we present a new form of microscopy, the frequency transmission electric-field (FTE) method using SEM, that offers low radiation damage and high-contrast observation of unstained biological samples in water. The wet biological specimens are enclosed in two silicon nitride (SiN) films. The metal-coated SiN film is irradiated using a focused modulation electron beam (EB) at a low-accelerating voltage. A measurement terminal under the sample holder detects the electric-field frequency signal, which contains structural information relating to the biological specimens. Our results in very little radiation damage to the sample, and the observation image is similar to the transmission image, depending on the sample volume. Our developed method can easily be utilized for the observation of various biological specimens in water.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0092780&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/19634
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

Files in This Item:

File Name/ File Size	Content Type	Version	Access	License
journal.pone.0092780.PDF（814KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Umezono, Tsukuba, Ibaraki, Japan

Recommended Citation:

Toshihiko Ogura. Direct Observation of Unstained Biological Specimens in Water by the Frequency Transmission Electric-Field Method Using SEM[J]. PLOS ONE,2014-01-01,9(3)

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