globalchange  > 过去全球变化的重建
DOI: 10.1371/journal.pone.0092141
论文题名:
Cation Diffusion Facilitators Transport Initiation and Regulation Is Mediated by Cation Induced Conformational Changes of the Cytoplasmic Domain
作者: Natalie Zeytuni; René Uebe; Michal Maes; Geula Davidov; Michal Baram; Oliver Raschdorf; Merav Nadav-Tsubery; Sofiya Kolusheva; Ronit Bitton; Gil Goobes; Assaf Friedler; Yifat Miller; Dirk Schüler; Raz Zarivach
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2014
发表日期: 2014-3-21
卷: 9, 期:3
语种: 英语
英文关键词: Cations ; Dimers (Chemical physics) ; Zinc ; Crystal structure ; Molecular dynamics ; Biochemical simulations ; Magnetite ; Simulation and modeling
英文摘要: Cation diffusion facilitators (CDF) are part of a highly conserved protein family that maintains cellular divalent cation homeostasis in all domains of life. CDF's were shown to be involved in several human diseases, such as Type-II diabetes and neurodegenerative diseases. In this work, we employed a multi-disciplinary approach to study the activation mechanism of the CDF protein family. For this we used MamM, one of the main ion transporters of magnetosomes – bacterial organelles that enable magnetotactic bacteria to orientate along geomagnetic fields. Our results reveal that the cytosolic domain of MamM forms a stable dimer that undergoes distinct conformational changes upon divalent cation binding. MamM conformational change is associated with three metal binding sites that were identified and characterized. Altogether, our results provide a novel auto-regulation mode of action model in which the cytosolic domain's conformational changes upon ligand binding allows the priming of the CDF into its transport mode.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0092141&type=printable
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/19905
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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作者单位: Department of Life Sciences, Ben Gurion University of the Negev, Beer-Sheva, Israel;National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer-Sheva, Israel;Ludwig Maximillian University of Munich, Department of Biology I, Martinsried, Germany;Institute of Chemistry, The Hebrew University of Jerusalem, Givat Ram, Jerusalem, Israel;Department of Life Sciences, Ben Gurion University of the Negev, Beer-Sheva, Israel;National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer-Sheva, Israel;Department of Chemistry, Ben Gurion University of the Negev, Beer-Sheva, Israel;Ilse Katz Institute for Nanoscale Science and Technology, Ben Gurion University of the Negev, Beer-Sheva, Israel;Ludwig Maximillian University of Munich, Department of Biology I, Martinsried, Germany;Department of Chemistry, Bar-Ilan University, Ramat Gan, Israel;Ilse Katz Institute for Nanoscale Science and Technology, Ben Gurion University of the Negev, Beer-Sheva, Israel;Ilse Katz Institute for Nanoscale Science and Technology, Ben Gurion University of the Negev, Beer-Sheva, Israel;Department of Chemical Engineering, Ben Gurion University of the Negev, Beer-Sheva, Israel;Department of Chemistry, Bar-Ilan University, Ramat Gan, Israel;Institute of Chemistry, The Hebrew University of Jerusalem, Givat Ram, Jerusalem, Israel;Ilse Katz Institute for Nanoscale Science and Technology, Ben Gurion University of the Negev, Beer-Sheva, Israel;Department of Chemical Engineering, Ben Gurion University of the Negev, Beer-Sheva, Israel;Ludwig Maximillian University of Munich, Department of Biology I, Martinsried, Germany;Department of Life Sciences, Ben Gurion University of the Negev, Beer-Sheva, Israel;National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer-Sheva, Israel

Recommended Citation:
Natalie Zeytuni,René Uebe,Michal Maes,et al. Cation Diffusion Facilitators Transport Initiation and Regulation Is Mediated by Cation Induced Conformational Changes of the Cytoplasmic Domain[J]. PLOS ONE,2014-01-01,9(3)
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