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globalchange > 过去全球变化的重建

DOI:	10.1371/journal.pone.0089922
论文题名:	Cultivation-Independent Screening Revealed Hot Spots of IncP-1, IncP-7 and IncP-9 Plasmid Occurrence in Different Environmental Habitats
作者:	Simone Dealtry; Guo-Chun Ding; Viola Weichelt; Vincent Dunon; Andreas Schlüter; María Carla Martini; María Florencia Del Papa; Antonio Lagares; Gregory Charles Auton Amos; Elizabeth Margaret Helen Wellington; William Hugo Gaze; Detmer Sipkema; Sara Sjöling; Dirk Springael; Holger Heuer; Jan Dirk van Elsas; Christopher Thomas; Kornelia Smalla
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2014
发表日期:	2014-2-24
卷:	9, 期:2
语种:	英语
英文关键词:	Sediment ; Edaphology ; Polymerase chain reaction ; Water pollution ; Baltic Sea ; Pesticides ; Pollutants ; Southern blot
英文摘要:	IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of plasmid-specific sequences in total community (TC-) DNA or fosmid DNA from samples originating from different environments and geographic regions. A novel primer system targeting IncP-9 plasmids was developed and applied along with established primers for IncP-1 and IncP-7. Screening TC-DNA from biopurification systems (BPS) which are used on farms for the purification of pesticide-contaminated water revealed high abundances of IncP-1 plasmids belonging to different subgroups as well as IncP-7 and IncP-9. The novel IncP-9 primer-system targeting the rep gene of nine IncP-9 subgroups allowed the detection of a high diversity of IncP-9 plasmid specific sequences in environments with different sources of pollution. Thus polluted sites are “hot spots” of plasmids potentially carrying catabolic genes.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089922&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/20126
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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File Name/ File Size	Content Type	Version	Access	License
journal.pone.0089922.PDF（650KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Julius Kühn-Institut – Federal Research Centre for Cultivated Plants (JKI), Institute for Epidemiology and Pathogen Diagnostics, Braunschweig, Germany;Julius Kühn-Institut – Federal Research Centre for Cultivated Plants (JKI), Institute for Epidemiology and Pathogen Diagnostics, Braunschweig, Germany;Julius Kühn-Institut – Federal Research Centre for Cultivated Plants (JKI), Institute for Epidemiology and Pathogen Diagnostics, Braunschweig, Germany;Division of Soil and Water Management, KU Leuven, Heverlee, Belgium;Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Bielefeld, Germany;IBBM (Instituto de Biotecnología y Biología Molecular), CCT-CONICET-La Plata, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina;IBBM (Instituto de Biotecnología y Biología Molecular), CCT-CONICET-La Plata, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina;IBBM (Instituto de Biotecnología y Biología Molecular), CCT-CONICET-La Plata, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina;School of Life Sciences, University of Warwick, Warwick, United Kingdom;School of Life Sciences, University of Warwick, Warwick, United Kingdom;School of Life Sciences, University of Warwick, Warwick, United Kingdom;Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands;Södertörns högskola (Sodertorn University), Inst. för Naturvetenskap, Miljö och medieteknik (School of Natural Sciences, Environmental Studies and media tech), Huddinge, Sweden;Division of Soil and Water Management, KU Leuven, Heverlee, Belgium;Julius Kühn-Institut – Federal Research Centre for Cultivated Plants (JKI), Institute for Epidemiology and Pathogen Diagnostics, Braunschweig, Germany;University of Groningen, Groningen, The Netherlands;School of Biosciences, University of Birmingham, Edgbaston, Birmingham, Warwick, United Kingdom;Julius Kühn-Institut – Federal Research Centre for Cultivated Plants (JKI), Institute for Epidemiology and Pathogen Diagnostics, Braunschweig, Germany

Recommended Citation:

Simone Dealtry,Guo-Chun Ding,Viola Weichelt,et al. Cultivation-Independent Screening Revealed Hot Spots of IncP-1, IncP-7 and IncP-9 Plasmid Occurrence in Different Environmental Habitats[J]. PLOS ONE,2014-01-01,9(2)

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