In the last decade, the aquatic eddy correlation (EC) technique has proven to be a powerful approach for non-invasive measurements of oxygen fluxes across the sediment water interface. Fundamental to the EC approach is the correlation of turbulent velocity and oxygen concentration fluctuations measured with high frequencies in the same sampling volume. Oxygen concentrations are commonly measured with fast responding electrochemical microsensors. However, due to their own oxygen consumption, electrochemical microsensors are sensitive to changes of the diffusive boundary layer surrounding the probe and thus to changes in the ambient flow velocity. The so-called stirring sensitivity of microsensors constitutes an inherent correlation of flow velocity and oxygen sensing and thus an artificial flux which can confound the benthic flux determination. To assess the artificial flux we measured the correlation between the turbulent flow velocity and the signal of oxygen microsensors in a sealed annular flume without any oxygen sinks and sources. Experiments revealed significant correlations, even for sensors designed to have low stirring sensitivities of ~0.7%. The artificial fluxes depended on ambient flow conditions and, counter intuitively, increased at higher velocities because of the nonlinear contribution of turbulent velocity fluctuations. The measured artificial fluxes ranged from 2 - 70 mmol m-2 d-1 for weak and very strong turbulent flow, respectively. Further, the stirring sensitivity depended on the sensor orientation towards the flow. For a sensor orientation typically used in field studies, the artificial flux could be predicted using a simplified mathematical model. Optical microsensors (optodes) that should not exhibit a stirring sensitivity were tested in parallel and did not show any significant correlation between O2 signals and turbulent flow. In conclusion, EC data obtained with electrochemical sensors can be affected by artificial flux and we recommend using optical microsensors in future EC-studies.
Max-Planck-Institute for Marine Microbiology, Biogeochemistry Group, Bremen, Germany;MARUM—Center for Marine Environmental Science, University of Bremen, Bremen, Germany;Institute for Environmental Sciences, University of Koblenz-Landau, Landau, Germany;University of Southern Denmark, Institute of Biology and Nordic Center for Earth Evolution (NordCEE), Odense, Denmark;Royal Netherland Institute for Sea Research (NIOZ), Yerseke, Netherlands;IGB—Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Experimental Limnology, Berlin, Germany;Institute F.-A. Forel, Faculty of Science, University of Geneva, Geneva, Switzerland;Institute for Environmental Sciences, University of Koblenz-Landau, Landau, Germany;University of Southern Denmark, Institute of Biology and Nordic Center for Earth Evolution (NordCEE), Odense, Denmark;Aarhus University, Arctic Research Center, Aarhus, Denmark;Scottish Association for Marine Science, Scottish Marine Institute, Oban, United Kingdom;Greenland Climate Research Centre, Greenland Institute of Natural Resources, Nuuk, Greenland
Recommended Citation:
Moritz Holtappels,Christian Noss,Kasper Hancke,et al. Aquatic Eddy Correlation: Quantifying the Artificial Flux Caused by Stirring-Sensitive O2 Sensors[J]. PLOS ONE,2015-01-01,10(1)