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DOI:	10.1371/journal.pone.0133207
论文题名:	Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus
作者:	María-Carmen Fernández-Pinos; Marta Casado; Gemma Caballero; Erik R. Zinser; Jordi Dachs; Benjamin Piña
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2015
发表日期:	2015-8-5
卷:	10, 期:8
语种:	英语
英文关键词:	Synechococcus ; Pollutants ; DNA filter assay ; Gene expression ; Sequence analysis ; DNA sequence analysis ; Photosynthesis ; Oceans
英文摘要:	Newly designed primers targeting rbcL (CO2 fixation), psbA (photosystem II) and rnpB (reference) genes were used in qRT-PCR assays to assess the photosynthetic capability of natural communities of Prochlorococcus, the most abundant photosynthetic organism on Earth and a major contributor to primary production in oligotrophic oceans. After optimizing sample collection methodology, we analyzed a total of 62 stations from the Malaspina 2010 circumnavigation (including Atlantic, Pacific and Indian Oceans) at three different depths. Sequence and quantitative analyses of the corresponding amplicons showed the presence of high-light (HL) and low-light (LL) Prochlorococcus clades in essentially all 182 samples, with a largely uniform stratification of LL and HL sequences. Synechococcus cross-amplifications were detected by the taxon-specific melting temperatures of the amplicons. Laboratory exposure of Prochlorococcus MED4 (HL) and MIT9313 (LL) strains to organic pollutants (PAHs and organochlorine compounds) showed a decrease of rbcL transcript abundances, and of the rbcL to psbA ratios for both strains. We propose this technique as a convenient assay to evaluate effects of environmental stressors, including pollution, on the oceanic Prochlorococcus photosynthetic function.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0133207&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/21108
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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File Name/ File Size	Content Type	Version	Access	License
journal.pone.0133207.PDF（3846KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Department of Environmental Chemistry, IDAEA-CSIC, Barcelona, Catalonia, Spain;Department of Environmental Chemistry, IDAEA-CSIC, Barcelona, Catalonia, Spain;Department of Environmental Chemistry, IDAEA-CSIC, Barcelona, Catalonia, Spain;Department of Microbiology, University of Tennessee, Knoxville, Tennessee, United States of America;Department of Environmental Chemistry, IDAEA-CSIC, Barcelona, Catalonia, Spain;Department of Environmental Chemistry, IDAEA-CSIC, Barcelona, Catalonia, Spain

Recommended Citation:

María-Carmen Fernández-Pinos,Marta Casado,Gemma Caballero,et al. Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus[J]. PLOS ONE,2015-01-01,10(8)

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