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DOI:	10.1371/journal.pone.0132642
论文题名:	Spontaneous Deletion of an “ORFanage” Region Facilitates Host Adaptation in a “Photosynthetic” Cyanophage
作者:	Richard J. Puxty; Blanca Perez-Sepulveda; Branko Rihtman; David J. Evans; Andrew D. Millard; David J. Scanlan
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2015
发表日期:	2015-7-15
卷:	10, 期:7
语种:	英语
英文关键词:	Metagenomics ; Bacteriophages ; Gene prediction ; Viral genomics ; Fresh water ; Polymerase chain reaction ; Comparative genomics ; Genome sequencing
英文摘要:	Viruses have been suggested to be the largest source of genetic diversity on Earth. Genome sequencing and metagenomic surveys reveal that novel genes with unknown functions are abundant in viral genomes. Yet few observations exist for the processes and frequency by which these genes are gained and lost. The surface waters of marine environments are dominated by marine picocyanobacteria and their co-existing viruses (cyanophages). Recent genome sequencing of cyanophages has revealed a vast array of genes that have been acquired from their cyanobacterial hosts. Here, we re-sequenced the cyanophage S-PM2 genome after 10 years of near continuous passage through its marine Synechococcus host. During this time a spontaneous mutant (S-PM2d) lacking 13% of the S-PM2 ORFs became dominant in the cyanophage population. These ORFs are found at one loci and are not homologous to any proteins in any other sequenced organism (ORFans). We demonstrate a fitness cost to S-PM2WT associated with possession of these ORFs under standard laboratory growth. Metagenomic surveys reveal these ORFs are present in various aquatic environments, are likely of cyanophage origin and appear to be enriched in environments from the extremes of salinity (freshwater and hypersaline). We posit that these ORFs contribute to the flexible gene content of cyanophages and offer a distinct fitness advantage in freshwater and hypersaline environments.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0132642&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/21147
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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journal.pone.0132642.PDF（6848KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

School of Life Sciences, University of Warwick, Coventry, West Midlands, CV4 7AL, United Kingdom;School of Life Sciences, University of Warwick, Coventry, West Midlands, CV4 7AL, United Kingdom;School of Life Sciences, University of Warwick, Coventry, West Midlands, CV4 7AL, United Kingdom;School of Life Sciences, University of Warwick, Coventry, West Midlands, CV4 7AL, United Kingdom;Warwick Medical School, University of Warwick, Coventry, West Midlands, CV4 7AL, United Kingdom;School of Life Sciences, University of Warwick, Coventry, West Midlands, CV4 7AL, United Kingdom

Recommended Citation:

Richard J. Puxty,Blanca Perez-Sepulveda,Branko Rihtman,et al. Spontaneous Deletion of an “ORFanage” Region Facilitates Host Adaptation in a “Photosynthetic” Cyanophage[J]. PLOS ONE,2015-01-01,10(7)

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