全球变化知识资源中心

Knowledge Resources Center for Global Change


Email:
Passwd
验证:
	换一张
Have you forgotten your password? Stay signed in Log In

globalchange > 过去全球变化的重建

DOI:	10.1371/journal.pone.0127593
论文题名:	Insights into Transcriptomes of Big and Low Sagebrush
作者:	Mark D. Huynh; Justin T. Page; Bryce A. Richardson; Joshua A. Udall
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2015
发表日期:	2015-5-28
卷:	10, 期:5
语种:	英语
英文关键词:	Terpenes ; Transcriptome analysis ; Paleogenetics ; Gene duplication ; Sequence alignment ; Evolutionary genetics ; Multiple alignment calculation ; Sequence assembly tools
英文摘要:	We report the sequencing and assembly of three transcriptomes from Big (Artemisia tridentata ssp. wyomingensis and A. tridentata ssp. tridentata) and Low (A. arbuscula ssp. arbuscula) sagebrush. The sequence reads are available in the Sequence Read Archive of NCBI. We demonstrate the utilities of these transcriptomes for gene discovery and phylogenomic analysis. An assembly of 61,883 transcripts followed by transcript identification by the program TRAPID revealed 16 transcripts directly related to terpene synthases, proteins critical to the production of multiple secondary metabolites in sagebrush. A putative terpene synthase was identified in two of our sagebrush samples. Using paralogs with synonymous mutations we reconstructed an evolutionary time line of ancient genome duplications. By applying a constant mutation rate to the data we estimate that these three ancient duplications occurred about 18, 34 and 60 million years ago. These transcriptomes offer a foundation for future studies of sagebrush, including inferences in chemical defense and the identification of species and subspecies of sagebrush for restoration and preservation of the threatened sage-grouse.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0127593&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/21258
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

Files in This Item:

File Name/ File Size	Content Type	Version	Access	License
journal.pone.0127593.PDF（763KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Plant and Wildlife Science Department, Brigham Young University, Provo, UT, 84602, United States of America;Plant and Wildlife Science Department, Brigham Young University, Provo, UT, 84602, United States of America;Rocky Mountain Research Station, USDA Forest Service, Provo, UT, 84606, United States of America;Plant and Wildlife Science Department, Brigham Young University, Provo, UT, 84602, United States of America

Recommended Citation:

Mark D. Huynh,Justin T. Page,Bryce A. Richardson,et al. Insights into Transcriptomes of Big and Low Sagebrush[J]. PLOS ONE,2015-01-01,10(5)

Service
	Recommend this item
	Sava as my favorate item
	Show this item's statistics
	Export Endnote File
Google Scholar
	Similar articles in Google Scholar
	[Mark D. Huynh]'s Articles
	[Justin T. Page]'s Articles
	[Bryce A. Richardson]'s Articles
百度学术
	Similar articles in Baidu Scholar
	[Mark D. Huynh]'s Articles
	[Justin T. Page]'s Articles
	[Bryce A. Richardson]'s Articles
CSDL cross search
	Similar articles in CSDL Cross Search
	[Mark D. Huynh]‘s Articles
	[Justin T. Page]‘s Articles
	[Bryce A. Richardson]‘s Articles
Related Copyright Policies
Null
收藏/分享