globalchange  > 过去全球变化的重建
DOI: 10.1371/journal.pone.0122616
论文题名:
Photosynthetic Membranes of Synechocystis or Plants Convert Sunlight to Photocurrent through Different Pathways due to Different Architectures
作者: Roy I. Pinhassi; Dan Kallmann; Gadiel Saper; Shirley Larom; Artyom Linkov; Alix Boulouis; Mark-Aurel Schöttler; Ralph Bock; Avner Rothschild; Noam Adir; Gadi Schuster
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2015
发表日期: 2015-4-27
卷: 10, 期:4
语种: 英语
英文关键词: Nicotiana ; Spinach ; Electron transfer ; Cyanobacteria ; Graphite ; Oxygen ; Photosynthesis ; Anodes
英文摘要: Thylakoid membranes contain the redox active complexes catalyzing the light-dependent reactions of photosynthesis in cyanobacteria, algae and plants. Crude thylakoid membranes or purified photosystems from different organisms have previously been utilized for generation of electrical power and/or fuels. Here we investigate the electron transferability from thylakoid preparations from plants or the cyanobacterium Synechocystis. We show that upon illumination, crude Synechocystis thylakoids can reduce cytochrome c. In addition, this crude preparation can transfer electrons to a graphite electrode, producing an unmediated photocurrent of 15 μA/cm2. Photocurrent could be obtained in the presence of the PSII inhibitor DCMU, indicating that the source of electrons is QA, the primary Photosystem II acceptor. In contrast, thylakoids purified from plants could not reduce cyt c, nor produced a photocurrent in the photocell in the presence of DCMU. The production of significant photocurrent (100 μA/cm2) from plant thylakoids required the addition of the soluble electron mediator DCBQ. Furthermore, we demonstrate that use of crude thylakoids from the D1-K238E mutant in Synechocystis resulted in improved electron transferability, increasing the direct photocurrent to 35 μA/cm2. Applying the analogous mutation to tobacco plants did not achieve an equivalent effect. While electron abstraction from crude thylakoids of cyanobacteria or plants is feasible, we conclude that the site of the abstraction of the electrons from the thylakoids, the architecture of the thylakoid preparations influence the site of the electron abstraction, as well as the transfer pathway to the electrode. This dictates the use of different strategies for production of sustainable electrical current from photosynthetic thylakoid membranes of cyanobacteria or higher plants.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0122616&type=printable
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/21317
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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作者单位: Grand Technion Energy Program, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Faculty of Biology, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Schulich Faculty of Chemistry, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Department of Science and Material Engineering, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Grand Technion Energy Program, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Faculty of Biology, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Schulich Faculty of Chemistry, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Department of Science and Material Engineering, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Grand Technion Energy Program, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Faculty of Biology, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Schulich Faculty of Chemistry, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Department of Science and Material Engineering, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Faculty of Biology, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Schulich Faculty of Chemistry, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Max-Planck-Institut für molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Potsdam-Golm, Germany;Max-Planck-Institut für molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Potsdam-Golm, Germany;Max-Planck-Institut für molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Potsdam-Golm, Germany;Grand Technion Energy Program, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Department of Science and Material Engineering, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Grand Technion Energy Program, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Schulich Faculty of Chemistry, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Grand Technion Energy Program, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel;Faculty of Biology, Technion—Israel Institute of Technology, Technion City, Haifa 32000 Israel

Recommended Citation:
Roy I. Pinhassi,Dan Kallmann,Gadiel Saper,et al. Photosynthetic Membranes of Synechocystis or Plants Convert Sunlight to Photocurrent through Different Pathways due to Different Architectures[J]. PLOS ONE,2015-01-01,10(4)
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