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globalchange > 过去全球变化的重建

DOI:	10.1371/journal.pone.0122934
论文题名:	Ultrastructure of the Interlamellar Membranes of the Nacre of the Bivalve Pteria hirundo, Determined by Immunolabelling
作者:	Antonio J. Osuna-Mascaró; Teresa Cruz-Bustos; Frédéric Marin; Antonio G. Checa
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2015
发表日期:	2015-4-24
卷:	10, 期:4
语种:	英语
英文关键词:	Chitin ; Proteases ; Bivalves ; Confocal microscopy ; Scanning electron microscopy ; Protein structure comparison ; Aragonite ; Molluscs
英文摘要:	The current model for the ultrastructure of the interlamellar membranes of molluscan nacre imply that they consist of a core of aligned chitin fibers surrounded on both sides by acidic proteins. This model was based on observations taken on previously demineralized shells, where the original structure had disappeared. Despite other earlier claims, no direct observations exist in which the different components can be unequivocally discriminated. We have applied different labeling protocols on non-demineralized nacreous shells of the bivalve Pteria. With this method, we have revealed the disposition and nature of the different fibers of the interlamellar membranes that can be observed on the surface of the nacreous shell of the bivalve Pteria hirundo by high resolution scanning electron microscopy (SEM). The minor chitin component consists of very thin fibers with a high aspect ratio and which are seemingly disoriented. Each fiber has a protein coat, which probably forms a complex with the chitin. The chitin-protein-complex fibers are embedded in an additional proteinaceous matrix. This is the first time in which the sizes, positions and distribution of the chitin fibers have been observed in situ.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0122934&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/21323
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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File Name/ File Size	Content Type	Version	Access	License
journal.pone.0122934.PDF（1143KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Departamento de Estratigrafía y Paleontología, Facultad de Ciencias, Universidad de Granada, Av. Fuentenueva, S/N 18071, Granada, Spain;Departamento de Bioquímica y Parasitología Molecular, Facultad de Ciencias, Universidad de Granada, Av. Fuentenueva, S/N 18071, Granada, Spain;UMR CNRS 6282 Biogéosciences, Université de Bourgogne, 6 Bd. Gabriel, 21000, Dijon, France;Departamento de Estratigrafía y Paleontología, Facultad de Ciencias, Universidad de Granada, Av. Fuentenueva, S/N 18071, Granada, Spain

Recommended Citation:

Antonio J. Osuna-Mascaró,Teresa Cruz-Bustos,Frédéric Marin,et al. Ultrastructure of the Interlamellar Membranes of the Nacre of the Bivalve Pteria hirundo, Determined by Immunolabelling[J]. PLOS ONE,2015-01-01,10(4)

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