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DOI: 10.1371/journal.pone.0121495
论文题名:
Pre-Analytical Sample Quality: Metabolite Ratios as an Intrinsic Marker for Prolonged Room Temperature Exposure of Serum Samples
作者: Gabriele Anton; Rory Wilson; Zhong-hao Yu; Cornelia Prehn; Sven Zukunft; Jerzy Adamski; Margit Heier; Christa Meisinger; Werner Römisch-Margl; Rui Wang-Sattler; Kristian Hveem; Bruce Wolfenbuttel; Annette Peters; Gabi Kastenmüller; Melanie Waldenberger
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2015
发表日期: 2015-3-30
卷: 10, 期:3
语种: 英语
英文关键词: Metabolites ; Ice core ; Amino acid metabolism ; Phenylalanine ; Metabolic processes ; Specimen storage ; Glycine ; Linear regression analysis
英文摘要: Advances in the “omics” field bring about the need for a high number of good quality samples. Many omics studies take advantage of biobanked samples to meet this need. Most of the laboratory errors occur in the pre-analytical phase. Therefore evidence-based standard operating procedures for the pre-analytical phase as well as markers to distinguish between ‘good’ and ‘bad’ quality samples taking into account the desired downstream analysis are urgently needed. We studied concentration changes of metabolites in serum samples due to pre-storage handling conditions as well as due to repeated freeze-thaw cycles. We collected fasting serum samples and subjected aliquots to up to four freeze-thaw cycles and to pre-storage handling delays of 12, 24 and 36 hours at room temperature (RT) and on wet and dry ice. For each treated aliquot, we quantified 127 metabolites through a targeted metabolomics approach. We found a clear signature of degradation in samples kept at RT. Storage on wet ice led to less pronounced concentration changes. 24 metabolites showed significant concentration changes at RT. In 22 of these, changes were already visible after only 12 hours of storage delay. Especially pronounced were increases in lysophosphatidylcholines and decreases in phosphatidylcholines. We showed that the ratio between the concentrations of these molecule classes could serve as a measure to distinguish between ‘good’ and ‘bad’ quality samples in our study. In contrast, we found quite stable metabolite concentrations during up to four freeze-thaw cycles. We concluded that pre-analytical RT handling of serum samples should be strictly avoided and serum samples should always be handled on wet ice or in cooling devices after centrifugation. Moreover, serum samples should be frozen at or below -80°C as soon as possible after centrifugation.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0121495&type=printable
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/21606
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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作者单位: Institute of Epidemiology II, Helmholtz Center Munich, Munich, Germany;Institute of Epidemiology II, Helmholtz Center Munich, Munich, Germany;Institute of Epidemiology II, Helmholtz Center Munich, Munich, Germany;Institute of Experimental Genetics, Helmholtz Center Munich, Munich, Germany;Institute of Experimental Genetics, Helmholtz Center Munich, Munich, Germany;Institute of Experimental Genetics, Helmholtz Center Munich, Munich, Germany;Institute of Epidemiology II, Helmholtz Center Munich, Munich, Germany;Central Hospital of Augsburg, KORA Myocardial Infarction Registry, Augsburg, Germany;Institute of Epidemiology II, Helmholtz Center Munich, Munich, Germany;Central Hospital of Augsburg, KORA Myocardial Infarction Registry, Augsburg, Germany;Institute for Bioinformatics and Systems Biology, Helmholtz Center Munich, Munich, Germany;Institute of Epidemiology II, Helmholtz Center Munich, Munich, Germany;Department of Public Health and General Practice, Norwegian University of Science and Technology, Levanger, Norway;Department of Endocrinology, University Medical Center Groningen, Groningen, The Netherlands;Institute of Epidemiology II, Helmholtz Center Munich, Munich, Germany;Institute for Bioinformatics and Systems Biology, Helmholtz Center Munich, Munich, Germany;Institute of Epidemiology II, Helmholtz Center Munich, Munich, Germany

Recommended Citation:
Gabriele Anton,Rory Wilson,Zhong-hao Yu,et al. Pre-Analytical Sample Quality: Metabolite Ratios as an Intrinsic Marker for Prolonged Room Temperature Exposure of Serum Samples[J]. PLOS ONE,2015-01-01,10(3)
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