globalchange  > 过去全球变化的重建
DOI: 10.1371/journal.pone.0136743
论文题名:
Vector-Host Interactions of Culiseta melanura in a Focus of Eastern Equine Encephalitis Virus Activity in Southeastern Virginia
作者: Goudarz Molaei; Philip M. Armstrong; Charles F. Abadam; Karen I. Akaratovic; Jay P. Kiser; Theodore G. Andreadis
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2015
发表日期: 2015-9-1
卷: 10, 期:9
语种: 英语
英文关键词: Birds ; Mosquitoes ; Blood ; Eastern equine encephalitis virus ; Passerines ; Swamps ; Polymerase chain reaction ; Starlings
英文摘要: Eastern equine encephalitis virus (EEEV) causes a highly pathogenic mosquito-borne zoonosis that is responsible for sporadic outbreaks of severe illness in humans and equines in the eastern USA. Culiseta (Cs.) melanura is the primary vector of EEEV in most geographic regions but its feeding patterns on specific avian and mammalian hosts are largely unknown in the mid-Atlantic region. The objectives of our study were to: 1) identify avian hosts of Cs. melanura and evaluate their potential role in enzootic amplification of EEEV, 2) assess spatial and temporal patterns of virus activity during a season of intense virus transmission, and 3) investigate the potential role of Cs. melanura in epidemic/epizootic transmission of EEEV to humans and equines. Accordingly, we collected mosquitoes at 55 sites in Suffolk, Virginia in 2013, and identified the source of blood meals in engorged mosquitoes by nucleotide sequencing PCR products of the mitochondrial cytochrome b gene. We also examined field-collected mosquitoes for evidence of infection with EEEV using Vector Test, cell culture, and PCR. Analysis of 188 engorged Cs. melanura sampled from April through October 2013 indicated that 95.2%, 4.3%, and 0.5% obtained blood meals from avian, mammalian, and reptilian hosts, respectively. American Robin was the most frequently identified host for Cs. melanura (42.6% of blood meals) followed by Northern Cardinal (16.0%), European Starling (11.2%), Carolina Wren (4.3%), and Common Grackle (4.3%). EEEV was detected in 106 mosquito pools of Cs. melanura, and the number of virus positive pools peaked in late July with 22 positive pools and a Maximum Likelihood Estimation (MLE) infection rate of 4.46 per 1,000 mosquitoes. Our findings highlight the importance of Cs. melanura as a regional EEEV vector based on frequent feeding on virus-competent bird species. A small proportion of blood meals acquired from mammalian hosts suggests the possibility that this species may occasionally contribute to epidemic/epizootic transmission of EEEV.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0136743&type=printable
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/21666
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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作者单位: Center for Vector Biology & Zoonotic Diseases, The Connecticut Agricultural Experiment Station, New Haven, Connecticut, United States of America;Center for Vector Biology & Zoonotic Diseases, The Connecticut Agricultural Experiment Station, New Haven, Connecticut, United States of America;Suffolk Mosquito Control, Department of Public Works, Suffolk, Virginia, United States of America;Suffolk Mosquito Control, Department of Public Works, Suffolk, Virginia, United States of America;Suffolk Mosquito Control, Department of Public Works, Suffolk, Virginia, United States of America;Center for Vector Biology & Zoonotic Diseases, The Connecticut Agricultural Experiment Station, New Haven, Connecticut, United States of America

Recommended Citation:
Goudarz Molaei,Philip M. Armstrong,Charles F. Abadam,et al. Vector-Host Interactions of Culiseta melanura in a Focus of Eastern Equine Encephalitis Virus Activity in Southeastern Virginia[J]. PLOS ONE,2015-01-01,10(9)
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