globalchange  > 过去全球变化的重建
DOI: 10.1371/journal.pone.0120839
论文题名:
What Do Pneumocystis Organisms Tell Us about the Phylogeography of Their Hosts? The Case of the Woodmouse Apodemus sylvaticus in Continental Europe and Western Mediterranean Islands
作者: Christine Demanche; Manjula Deville; Johan Michaux; Véronique Barriel; Claire Pinçon; Cécile Marie Aliouat-Denis; Muriel Pottier; Christophe Noël; Eric Viscogliosi; El Moukhtar Aliouat; Eduardo Dei-Cas; Serge Morand; Jacques Guillot
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2015
发表日期: 2015-4-1
卷: 10, 期:4
语种: 英语
英文关键词: Pneumocystis ; Phylogeography ; Host-pathogen interactions ; Ribosomal RNA ; Phylogenetic analysis ; Polymerase chain reaction ; Population genetics ; Sequence alignment
英文摘要: Pneumocystis fungi represent a highly diversified biological group with numerous species, which display a strong host-specificity suggesting a long co-speciation process. In the present study, the presence and genetic diversity of Pneumocystis organisms was investigated in 203 lung samples from woodmice (Apodemus sylvaticus) collected on western continental Europe and Mediterranean islands. The presence of Pneumocystis DNA was assessed by nested PCR at both large and small mitochondrial subunit (mtLSU and mtSSU) rRNA loci. Direct sequencing of nested PCR products demonstrated a very high variability among woodmouse-derived Pneumocystis organisms with a total number of 30 distinct combined mtLSU and mtSSU sequence types. However, the genetic divergence among these sequence types was very low (up to 3.87%) and the presence of several Pneumocystis species within Apodemus sylvaticus was considered unlikely. The analysis of the genetic structure of woodmouse-derived Pneumocystis revealed two distinct groups. The first one comprised Pneumocystis from woodmice collected in continental Spain, France and Balearic islands. The second one included Pneumocystis from woodmice collected in continental Italy, Corsica and Sicily. These two genetic groups were in accordance with the two lineages currently described within the host species Apodemus sylvaticus. Pneumocystis organisms are emerging as powerful tools for phylogeographic studies in mammals.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0120839&type=printable
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/21701
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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作者单位: Laboratoire de Parasitologie (EA4547), Faculté de Pharmacie, Université de Lille, Lille, France;Institut Pasteur de Lille, Centre d’Infection et d’Immunité de Lille, Inserm U1019, UMR CNRS 8204, Université de Lille, BioPôle d'Alfort, Biologie et Diversité des Pathogènes Eucaryotes Emergents, Lille, France;ENVA, UPEC, Research group Dynamyc, Ecole Nationale Vétérinaire d’Alfort, 94704, Maisons-Alfort Cedex, France;CBGP (Centre de Biologie et de Gestion des Populations), UMR INRA/IRD/Cirad/Montpellier SupAgro, Campus international de Baillarguet, CS 30016, 34988, Montferrier-sur-Lez cedex, France;Institut de Botanique (B22), University of Liège, 4000, Liège, (Sart Tilman), Belgium;Muséum national d’histoire naturelle, CR2P—UMR 7207 CNRS, MNHN, Univ Paris06, Paris, France;Departement of Biostatistics (EA2694), Université de Lille, Lille, France;Laboratoire de Parasitologie (EA4547), Faculté de Pharmacie, Université de Lille, Lille, France;Institut Pasteur de Lille, Centre d’Infection et d’Immunité de Lille, Inserm U1019, UMR CNRS 8204, Université de Lille, BioPôle d'Alfort, Biologie et Diversité des Pathogènes Eucaryotes Emergents, Lille, France;Laboratoire de Parasitologie (EA4547), Faculté de Pharmacie, Université de Lille, Lille, France;Institut Pasteur de Lille, Centre d’Infection et d’Immunité de Lille, Inserm U1019, UMR CNRS 8204, Université de Lille, BioPôle d'Alfort, Biologie et Diversité des Pathogènes Eucaryotes Emergents, Lille, France;Geneius Laboratories Ltd, INEX Business Centre, Newcastle upon Tyne, United Kingdom;Institut Pasteur de Lille, Centre d’Infection et d’Immunité de Lille, Inserm U1019, UMR CNRS 8204, Université de Lille, BioPôle d'Alfort, Biologie et Diversité des Pathogènes Eucaryotes Emergents, Lille, France;Laboratoire de Parasitologie (EA4547), Faculté de Pharmacie, Université de Lille, Lille, France;Institut Pasteur de Lille, Centre d’Infection et d’Immunité de Lille, Inserm U1019, UMR CNRS 8204, Université de Lille, BioPôle d'Alfort, Biologie et Diversité des Pathogènes Eucaryotes Emergents, Lille, France;Institut Pasteur de Lille, Centre d’Infection et d’Immunité de Lille, Inserm U1019, UMR CNRS 8204, Université de Lille, BioPôle d'Alfort, Biologie et Diversité des Pathogènes Eucaryotes Emergents, Lille, France;Parasitologie-Mycologie (EA4547) Faculté de Médecine, Université de Lille, CHRU, Lille, France;Institut des Sciences de l’Evolution, UMR CNRS-IRD-UM2, Université de Montpellier 2, F-34093, Montpellier, France;CIRAD-CNRS, Centre d’Infectiologie Christophe Mérieux du Laos, Vientiane, Lao PDR;ENVA, UPEC, Research group Dynamyc, Ecole Nationale Vétérinaire d’Alfort, 94704, Maisons-Alfort Cedex, France

Recommended Citation:
Christine Demanche,Manjula Deville,Johan Michaux,et al. What Do Pneumocystis Organisms Tell Us about the Phylogeography of Their Hosts? The Case of the Woodmouse Apodemus sylvaticus in Continental Europe and Western Mediterranean Islands[J]. PLOS ONE,2015-01-01,10(4)
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