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DOI:	10.1371/journal.pone.0135337
论文题名:	Rapid Characterization of Microalgae and Microalgae Mixtures Using Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS)
作者:	Duane Barbano; Regina Diaz; Lin Zhang; Todd Sandrin; Henri Gerken; Thomas Dempster
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2015
发表日期:	2015-8-13
卷:	10, 期:8
语种:	英语
英文关键词:	Matrix-assisted laser desorption ionization time-of-flight mass spectrometry ; Fresh water ; Mass spectra ; Sequence analysis ; Chlamydomonas reinhardtii ; Taxonomy ; Marine biology ; Protein extraction
英文摘要:	Current molecular methods to characterize microalgae are time-intensive and expensive. Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) may represent a rapid and economical alternative approach. The objectives of this study were to determine whether MALDI-TOF MS can be used to: 1) differentiate microalgae at the species and strain levels and 2) characterize simple microalgal mixtures. A common protein extraction sample preparation method was used to facilitate rapid mass spectrometry-based analysis of 31 microalgae. Each yielded spectra containing between 6 and 56 peaks in the m/z 2,000 to 20,000 range. The taxonomic resolution of this approach appeared higher than that of 18S rDNA sequence analysis. For example, two strains of Scenedesmus acutus differed only by two 18S rDNA nucleotides, but yielded distinct MALDI-TOF mass spectra. Mixtures of two and three microalgae yielded relatively complex spectra that contained peaks associated with members of each mixture. Interestingly, though, mixture-specific peaks were observed at m/z 11,048 and 11,230. Our results suggest that MALDI-TOF MS affords rapid characterization of individual microalgae and simple microalgal mixtures.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0135337&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/21866
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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File Name/ File Size	Content Type	Version	Access	License
journal.pone.0135337.PDF（1702KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

School of Life Sciences, Arizona State University, 427 East Tyler Mall, Tempe, Arizona, United States of America;School of Life Sciences, Arizona State University, 427 East Tyler Mall, Tempe, Arizona, United States of America;School of Mathematical and Natural Sciences, Arizona State University, MC 2352, P.O. Box 37100, Phoenix, Arizona, United States of America;School of Mathematical and Natural Sciences, Arizona State University, MC 2352, P.O. Box 37100, Phoenix, Arizona, United States of America;Arizona Center for Algae Technology and Innovation, Arizona State University, 7418 Innovation Way South, Building ISTB-3, Room 103, Mesa, Arizona, United States of America;Arizona Center for Algae Technology and Innovation, Arizona State University, 7418 Innovation Way South, Building ISTB-3, Room 103, Mesa, Arizona, United States of America

Recommended Citation:

Duane Barbano,Regina Diaz,Lin Zhang,et al. Rapid Characterization of Microalgae and Microalgae Mixtures Using Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS)[J]. PLOS ONE,2015-01-01,10(8)

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