globalchange  > 过去全球变化的重建
DOI: 10.1371/journal.pone.0130934
论文题名:
Seasonal Variation in the Skin Transcriptome of Common Bottlenose Dolphins (Tursiops truncatus) from the Northern Gulf of Mexico
作者: Frances M. Van Dolah; Marion G. Neely; Lauren E. McGeorge; Brian C. Balmer; Gina M. Ylitalo; Eric S. Zolman; Todd Speakman; Carrie Sinclair; Nicholas M. Kellar; Patricia E. Rosel; Keith D. Mullin; Lori H. Schwacke
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2015
发表日期: 2015-6-25
卷: 10, 期:6
语种: 英语
英文关键词: Dolphins ; Gene expression ; Contaminants ; Microarrays ; Biopsy ; Lipids ; Epidermis ; Summer
英文摘要: As long-lived predators that integrate exposures across multiple trophic levels, cetaceans are recognized as sentinels for the health of marine ecosystems. Their utility as sentinels requires the establishment of baseline health parameters. Because cetaceans are protected, measurements obtained with minimal disruption to free ranging animals are highly desirable. In this study we investigated the utility of skin gene expression profiling to monitor health and contaminant exposure in common bottlenose dolphins (Tursiops truncatus). Remote integument biopsies were collected in the northern Gulf of Mexico prior to the Deepwater Horizon oil spill (May 2010) and during summer and winter for two years following oil contamination (2010-2011). A bottlenose dolphin microarray was used to characterize the skin transcriptomes of 94 individuals from three populations: Barataria Bay, Louisiana, Chandeleur Sound, Louisiana, and Mississippi Sound, Mississippi/Alabama. Skin transcriptomes did not differ significantly between populations. In contrast, season had a profound effect on gene expression, with nearly one-third of all genes on the array differing in expression between winter and the warmer seasons (moderated T-test; p<0.01, fold-change≥1.5). Persistent organic pollutants (POPs) in blubber changed concurrently, reaching >two-fold higher concentrations in summer compared to winter, due to a seasonal decrease in blubber thickness and loss of stored lipid. However, global gene expression did not correlate strongly with seasonally changing contaminant concentrations, most likely because the refractory, lipid-stored metabolites are not substrates for phase I or II xenobiotic detoxification pathways. Rather, processes related to cell proliferation, motility, and differentiation dominated the differences in expression in winter and the warmer seasons. More subtle differences were seen between spring and summer (1.5% of genes differentially expressed). However, two presumed oil-exposed animals from spring presented gene expression profiles more similar to the summer animals (presumed exposed) than to other spring animals. Seasonal effects have not previously been considered in studies assessing gene expression in cetaceans, but clearly must be taken into account when applying transcriptomic analyses to investigate their contaminant exposure or health status.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0130934&type=printable
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/21917
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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作者单位: National Centers for Coastal Ocean Science, National Ocean Service, National Oceanic and Atmospheric Administration, Charleston, South Carolina, United States of America;National Centers for Coastal Ocean Science, National Ocean Service, National Oceanic and Atmospheric Administration, Charleston, South Carolina, United States of America;National Centers for Coastal Ocean Science, National Ocean Service, National Oceanic and Atmospheric Administration, Charleston, South Carolina, United States of America;National Centers for Coastal Ocean Science, National Ocean Service, National Oceanic and Atmospheric Administration, Charleston, South Carolina, United States of America;Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, Seattle, Washington, United States of America;National Centers for Coastal Ocean Science, National Ocean Service, National Oceanic and Atmospheric Administration, Charleston, South Carolina, United States of America;National Centers for Coastal Ocean Science, National Ocean Service, National Oceanic and Atmospheric Administration, Charleston, South Carolina, United States of America;Southeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, Pascagoula, Mississippi, United States of America;Southwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, La Jolla, California, United States of America;Southeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, Lafayette, Louisiana, United States of America;Southeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, Pascagoula, Mississippi, United States of America;National Centers for Coastal Ocean Science, National Ocean Service, National Oceanic and Atmospheric Administration, Charleston, South Carolina, United States of America

Recommended Citation:
Frances M. Van Dolah,Marion G. Neely,Lauren E. McGeorge,et al. Seasonal Variation in the Skin Transcriptome of Common Bottlenose Dolphins (Tursiops truncatus) from the Northern Gulf of Mexico[J]. PLOS ONE,2015-01-01,10(6)
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