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DOI:	10.1371/journal.pone.0130965
论文题名:	Development and Validation of Environmental DNA (eDNA) Markers for Detection of Freshwater Turtles
作者:	Christina M. Davy; Anne G. Kidd; Chris C. Wilson
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2015
发表日期:	2015-7-22
卷:	10, 期:7
语种:	英语
英文关键词:	Turtles ; Polymerase chain reaction ; Invasive species ; Survey methods ; Target detection ; Conservation science ; DNA extraction ; Ponds
英文摘要:	Environmental DNA (eDNA) is a potentially powerful tool for detection and monitoring of rare species, including threatened native species and recently arrived invasive species. Here, we develop DNA primers for a suite of nine sympatric freshwater turtles, and use it to test whether turtle eDNA can be successfully detected in samples from aquaria and an outdoor pond. We also conduct a cost comparison between eDNA detection and detection through traditional survey methods, using data from field surveys at two sites in our target area. We find that eDNA from turtles can be detected using both conventional polymerase chain reaction (PCR) and quantitative PCR (qPCR), and that the cost of detection through traditional survey methods is 2–10X higher than eDNA detection for the species in our study range. We summarize necessary future steps for application of eDNA surveys to turtle monitoring and conservation and propose specific cases in which the application of eDNA could further the conservation of threatened turtle species.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0130965&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/22204
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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File Name/ File Size	Content Type	Version	Access	License
journal.pone.0130965.PDF（1647KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Wildlife Preservation Canada, Guelph, Ontario, Canada;Trent University, Peterborough, Ontario, Canada;Ontario Ministry of Natural Resources and Forestry, Peterborough, Ontario, Canada;Ontario Ministry of Natural Resources and Forestry, Peterborough, Ontario, Canada

Recommended Citation:

Christina M. Davy,Anne G. Kidd,Chris C. Wilson. Development and Validation of Environmental DNA (eDNA) Markers for Detection of Freshwater Turtles[J]. PLOS ONE,2015-01-01,10(7)

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