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DOI:	10.1371/journal.pone.0129322
论文题名:	Inhibitory Effect of Aqueous Extracts from Marine Sponges on the Activity and Expression of Gelatinases A (MMP-2) and B (MMP-9) in Rat Astrocyte Cultures
作者:	Gaetano Di Bari; Eugenia Gentile; Tiziana Latronico; Giuseppe Corriero; Anna Fasano; Carlotta Nonnis Marzano; Grazia Maria Liuzzi
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2015
发表日期:	2015-6-8
卷:	10, 期:6
语种:	英语
英文关键词:	Astrocytes ; Sponges ; Protein extraction ; Marine biology ; Reverse transcriptase-polymerase chain reaction ; RNA extraction ; Specimen preparation and treatment ; Drug research and development
英文摘要:	The aim of this study was to evaluate whether water soluble compounds present in aqueous extracts from seven Mediterranean demosponges exert biological activity towards matrix metalloproteinases (MMPs), which represent important pathogenic factors of human diseases. Aqueous extracts were tested on LPS-activated cultured rat astrocytes, and levels and expression of MMP-2 and MMP-9 were assessed by zymography and RT-PCR, respectively. Our results demonstrated that the studied extracts contain water soluble compounds able to inhibit MMP-2 and MMP-9 activity and expression. We also compared the anti-MMP activities present in aqueous extracts from wild and reared specimens of Tethya aurantium and T. citrina. The results obtained revealed that the reared sponges maintain the production of bioactive compounds with inhibitory effect on MMP-2 and MMP-9 for all the duration of the rearing period. Taken together, our results indicate that the aqueous extracts from the selected Mediterranean demosponges possess a variety of water-soluble bioactive compounds, which are able to inhibit MMPs at different levels. The presence of biological activity in aqueous extracts from reared specimens of T. aurantium and T. citrina strongly encourage sponge aquaculture as a valid option to supply sponge biomass for drug development on a large scale.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0129322&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/22251
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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File Name/ File Size	Content Type	Version	Access	License
journal.pone.0129322.PDF（1617KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Department of Biosciences, Biotechnologies and Biopharmaceutics, Aldo Moro University, Bari, Italy;Department of Biosciences, Biotechnologies and Biopharmaceutics, Aldo Moro University, Bari, Italy;Department of Biosciences, Biotechnologies and Biopharmaceutics, Aldo Moro University, Bari, Italy;Department of Biology, Aldo Moro University, Bari, Italy;Department of Biosciences, Biotechnologies and Biopharmaceutics, Aldo Moro University, Bari, Italy;Department of Biology, Aldo Moro University, Bari, Italy;Department of Biosciences, Biotechnologies and Biopharmaceutics, Aldo Moro University, Bari, Italy

Recommended Citation:

Gaetano Di Bari,Eugenia Gentile,Tiziana Latronico,et al. Inhibitory Effect of Aqueous Extracts from Marine Sponges on the Activity and Expression of Gelatinases A (MMP-2) and B (MMP-9) in Rat Astrocyte Cultures[J]. PLOS ONE,2015-01-01,10(6)

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