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DOI:	10.1371/journal.pone.0128267
论文题名:	Hunting the Extinct Steppe Bison (Bison priscus) Mitochondrial Genome in the Trois-Frères Paleolithic Painted Cave
作者:	Marie-Claude Marsolier-Kergoat; Pauline Palacio; Véronique Berthonaud; Frédéric Maksud; Thomas Stafford; Robert Bégouën; Jean-Marc Elalouf
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2015
发表日期:	2015-6-17
卷:	10, 期:6
语种:	英语
英文关键词:	Bison ; Mitochondria ; Polymerase chain reaction ; Sequence assembly tools ; Sequence alignment ; Paleogenetics ; Genomic libraries ; Ancient DNA
英文摘要:	Despite the abundance of fossil remains for the extinct steppe bison (Bison priscus), an animal that was painted and engraved in numerous European Paleolithic caves, a complete mitochondrial genome sequence has never been obtained for this species. In the present study we collected bone samples from a sector of the Trois-Frères Paleolithic cave (Ariège, France) that formerly functioned as a pitfall and was sealed before the end of the Pleistocene. Screening the DNA content of the samples collected from the ground surface revealed their contamination by Bos DNA. However, a 19,000-year-old rib collected on a rock apart the pathway delineated for modern visitors was devoid of such contaminants and reproducibly yielded Bison priscus DNA. High-throughput shotgun sequencing combined with conventional PCR analysis of the rib DNA extract enabled to reconstruct a complete mitochondrial genome sequence of 16,318 bp for the extinct steppe bison with a 10.4-fold coverage. Phylogenetic analyses robustly established the position of the Bison priscus mitochondrial genome as basal to the clade delineated by the genomes of the modern American Bison bison. The extinct steppe bison sequence, which exhibits 93 specific polymorphisms as compared to the published Bison bison mitochondrial genomes, provides an additional resource for the study of Bovinae specimens. Moreover this study of ancient DNA delineates a new research pathway for the analysis of the Magdalenian Trois-Frères cave.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0128267&type=printable
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资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/22399
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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作者单位:

iBiTec-S/SBiGeM, CEA/Saclay 91191 Gif-sur-Yvette cedex, France;CNRS-UMR7206, Eco-anthropologie et Ethnobiologie, Département Hommes, Natures et Sociétés, Musée de l’Homme, 17 place du Trocadéro, 75016 Paris, France;iBiTec-S/SBiGeM, CEA/Saclay 91191 Gif-sur-Yvette cedex, France;CNRS-UMR7206, Eco-anthropologie et Ethnobiologie, Département Hommes, Natures et Sociétés, Musée de l’Homme, 17 place du Trocadéro, 75016 Paris, France;iBiTec-S/SBiGeM, CEA/Saclay 91191 Gif-sur-Yvette cedex, France;Service Régional de l’Archéologie, 32 rue de la Dalbade, BP811 31080 Toulouse cedex 6, France;AMS 14C Dating Centre, Department of Physics and Astronomy, University of Aarhus, Ny Munkegade 120, Aarhus, Denmark;Association Louis Bégouën, Laboratoire de Préhistoire de Pujol, 09200 Montesquieu-Avantès, France;iBiTec-S/SBiGeM, CEA/Saclay 91191 Gif-sur-Yvette cedex, France;CNRS-UMR7206, Eco-anthropologie et Ethnobiologie, Département Hommes, Natures et Sociétés, Musée de l’Homme, 17 place du Trocadéro, 75016 Paris, France

Recommended Citation:

Marie-Claude Marsolier-Kergoat,Pauline Palacio,Véronique Berthonaud,et al. Hunting the Extinct Steppe Bison (Bison priscus) Mitochondrial Genome in the Trois-Frères Paleolithic Painted Cave[J]. PLOS ONE,2015-01-01,10(6)

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