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DOI:	10.1371/journal.pone.0154610
论文题名:	A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids
作者:	Diana Massai; Giuseppe Isu; Denise Madeddu; Giulia Cerino; Angela Falco; Caterina Frati; Diego Gallo; Marco A. Deriu; Giuseppe Falvo D’Urso Labate; Federico Quaini; Alberto Audenino; Umberto Morbiducci
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2016
发表日期:	2016-5-4
卷:	11, 期:5
语种:	英语
英文关键词:	Shear stresses ; Flow rate ; Cell cultures ; Suspension cultures ; Apoptosis ; Dissolved oxygen ; Sedimentation ; Biological transport
英文摘要:	A versatile bioreactor suitable for dynamic suspension cell culture under tunable shear stress conditions has been developed and preliminarily tested culturing cancer cell spheroids. By adopting simple technological solutions and avoiding rotating components, the bioreactor exploits the laminar hydrodynamics establishing within the culture chamber enabling dynamic cell suspension in an environment favourable to mass transport, under a wide range of tunable shear stress conditions. The design phase of the device has been supported by multiphysics modelling and has provided a comprehensive analysis of the operating principles of the bioreactor. Moreover, an explanatory example is herein presented with multiphysics simulations used to set the proper bioreactor operating conditions for preliminary in vitro biological tests on a human lung carcinoma cell line. The biological results demonstrate that the ultralow shear dynamic suspension provided by the device is beneficial for culturing cancer cell spheroids. In comparison to the static suspension control, dynamic cell suspension preserves morphological features, promotes intercellular connection, increases spheroid size (2.4-fold increase) and number of cycling cells (1.58-fold increase), and reduces double strand DNA damage (1.5-fold reduction). It is envisioned that the versatility of this bioreactor could allow investigation and expansion of different cell types in the future.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0154610&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/23214
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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File Name/ File Size	Content Type	Version	Access	License
journal.pone.0154610.PDF（1309KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Turin, Italy;Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Turin, Italy;Department of Clinical and Experimental Medicine, Università degli Studi di Parma, Parma, Italy;Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Turin, Italy;Department of Clinical and Experimental Medicine, Università degli Studi di Parma, Parma, Italy;Department of Clinical and Experimental Medicine, Università degli Studi di Parma, Parma, Italy;Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Turin, Italy;Istituto Dalle Molle di studi sull'Intelligenza Artificiale, Scuola universitaria professionale della Svizzera italiana, Università della Svizzera italiana, Manno, Switzerland;Bioexpansys Srl, Turin, Italy;Department of Clinical and Experimental Medicine, Università degli Studi di Parma, Parma, Italy;Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Turin, Italy;Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Turin, Italy

Recommended Citation:

Diana Massai,Giuseppe Isu,Denise Madeddu,et al. A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids[J]. PLOS ONE,2016-01-01,11(5)

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