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DOI:	10.1371/journal.pone.0167105
论文题名:	From Lab to Lake – Evaluation of Current Molecular Methods for the Detection of Infectious Enteric Viruses in Complex Water Matrices in an Urban Area
作者:	Mats Leifels; Ibrahim Ahmed Hamza; Marion Krieger; Michael Wilhelm; Martin Mackowiak; Lars Jurzik
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2016
发表日期:	2016-11-23
卷:	11, 期:11
语种:	英语
英文关键词:	Sewage ; Cell cultures ; Surface water ; Sewage treatment ; Rotavirus infection ; Enterovirus infection ; Viral diseases ; Adenoviruses
英文摘要:	Quantitative PCR methods are commonly used to monitor enteric viruses in the aquatic environment because of their high sensitivity, short reaction times and relatively low operational cost. However, conclusions for public health drawn from results of such molecular techniques are limited due to their inability to determine viral infectivity. Ethidium monoazide (EMA) and propidium monoazide (PMA) are capable to penetrate the damaged or compromised capsid of the inactivated viruses and bind to the viral nucleic acids. We assessed whether dye treatment is a suitable approach to improve the ability of qPCR to distinguish between infectious and non-infectious human adenovirus, enterovirus and rotavirus A in surface water of an urban river and sewage before and after UV disinfection. Like the gold standard of cell culture assays, pretreatment EMA-/PMA-qPCR succeeded in removing false positive results which would lead to an overestimation of the viral load if only qPCR of the environmental samples was considered. A dye pretreatment could therefore provide a rapid and relatively inexpensive tool to improve the efficacy of molecular quantification methods in regards to viral infectivity.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0167105&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/23426
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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File Name/ File Size	Content Type	Version	Access	License
journal.pone.0167105.pdf（2357KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Ruhr-University Bochum, Department of Hygiene, Social- and Environmental Medicine, Bochum, Germany;Environmental Virology Laboratory, Department of Water Pollution Research, National Research Centre, Cairo, Egypt;Ruhr-University Bochum, Department of Hygiene, Social- and Environmental Medicine, Bochum, Germany;Ruhr-University Bochum, Department of Hygiene, Social- and Environmental Medicine, Bochum, Germany;University of Duisburg-Essen, Faculty of Chemistry, Biofilm Centre – Aquatic Microbiology, Essen, Germany;Ruhr-University Bochum, Department of Hygiene, Social- and Environmental Medicine, Bochum, Germany

Recommended Citation:

Mats Leifels,Ibrahim Ahmed Hamza,Marion Krieger,et al. From Lab to Lake – Evaluation of Current Molecular Methods for the Detection of Infectious Enteric Viruses in Complex Water Matrices in an Urban Area[J]. PLOS ONE,2016-01-01,11(11)

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