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DOI:	10.1371/journal.pone.0158213
论文题名:	Experimental Phage Therapy for Burkholderia pseudomallei Infection
作者:	Ong Guang-Han; Choh Leang-Chung; Kumutha Malar Vellasamy; Vanitha Mariappan; Chang Li-Yen; Jamuna Vadivelu
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2016
发表日期:	2016-7-7
卷:	11, 期:7
语种:	英语
英文关键词:	Bacteriophages ; Spleen ; Mouse models ; Respiratory infections ; Antibiotics ; Burkholderia infection ; Sea water ; Transmission electron microscopy
英文摘要:	Burkholderia pseudomallei is an intracellular Gram-negative bacterial pathogen intrinsically resistant to a variety of antibiotics. Phages have been developed for use as an alternative treatment therapy, particularly for bacterial infections that do not respond to conventional antibiotics. In this study, we investigated the use of phages to treat cells infected with B. pseudomallei. Phage C34 isolated from seawater was purified and characterised on the basis of its host range and morphology using transmission electron microscopy (TEM). Phage C34 was able to lyse 39.5% of B. pseudomallei clinical strains. Due to the presence of contractile tail, phage C34 is classified as a member of the family Myoviridae, a tailed double-stranded DNA virus. When 2 × 105 A549 cells were exposed to 2 × 107 PFU of phage C34, 24 hours prior to infection with 2 × 106 CFU of B. pseudomallei, it was found that the survivability of the cells increased to 41.6 ± 6.8% as compared to 22.8 ± 6.0% in untreated control. Additionally, application of phage successfully rescued 33.3% of mice infected with B. pseudomallei and significantly reduced the bacterial load in the spleen of the phage-treated mice. These findings indicate that phage can be a potential antimicrobial agent for B. pseudomallei infections.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0158213&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/23586
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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File Name/ File Size	Content Type	Version	Access	License
journal.pone.0158213.PDF（1790KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya, Kuala Lumpur, Malaysia;Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

Recommended Citation:

Ong Guang-Han,Choh Leang-Chung,Kumutha Malar Vellasamy,et al. Experimental Phage Therapy for Burkholderia pseudomallei Infection[J]. PLOS ONE,2016-01-01,11(7)

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