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DOI: 10.1371/journal.pone.0153500
论文题名:
Detection of Adult Green Sturgeon Using Environmental DNA Analysis
作者: Paul S. Bergman; Gregg Schumer; Scott Blankenship; Elizabeth Campbell
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2016
发表日期: 2016-4-20
卷: 11, 期:4
语种: 英语
英文关键词: Sturgeons ; Spawning ; DNA extraction ; Habitats ; Polymerase chain reaction ; Surface water ; Survey methods ; DNA analysis
英文摘要: Environmental DNA (eDNA) is an emerging sampling method that has been used successfully for detection of rare aquatic species. The Identification of sampling tools that are less stressful for target organisms has become increasingly important for rare and endangered species. A decline in abundance of the Southern Distinct Population Segment (DPS) of North American Green Sturgeon located in California’s Central Valley has led to its listing as Threatened under the Federal Endangered Species Act in 2006. While visual surveys of spawning Green Sturgeon in the Central Valley are effective at monitoring fish densities in concentrated pool habitats, results do not scale well to the watershed level, providing limited spatial and temporal context. Unlike most traditional survey methods, environmental DNA analysis provides a relatively quick, inexpensive tool that could efficiently monitor the presence and distribution of aquatic species. We positively identified Green Sturgeon DNA at two locations of known presence in the Sacramento River, proving that eDNA can be effective for monitoring the presence of adult sturgeon. While further study is needed to understand uncertainties of the sampling method, our study represents the first documented detection of Green Sturgeon eDNA, indicating that eDNA analysis could provide a new tool for monitoring Green Sturgeon distribution in the Central Valley, complimenting traditional on-going survey methods.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0153500&type=printable
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/23688
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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作者单位: Cramer Fish Sciences, West Sacramento, California, United States of America;Cramer Fish Sciences, West Sacramento, California, United States of America;Cramer Fish Sciences, West Sacramento, California, United States of America;United States Fish and Wildlife Service, Anadromous Fish Restoration Program, Lodi, Caifornia, United States of America

Recommended Citation:
Paul S. Bergman,Gregg Schumer,Scott Blankenship,et al. Detection of Adult Green Sturgeon Using Environmental DNA Analysis[J]. PLOS ONE,2016-01-01,11(4)
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