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DOI:	10.1371/journal.pone.0167099
论文题名:	Genetic Diversity of Blumeria graminis f. sp. hordei in Central Europe and Its Comparison with Australian Population
作者:	Eva Komínková; Antonín Dreiseitl; Eva Malečková; Jaroslav Doležel; Miroslav Valárik
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2016
发表日期:	2016-11-22
卷:	11, 期:11
语种:	英语
英文关键词:	Population genetics ; Barley ; Phylogenetic analysis ; Genetic polymorphism ; Polymerase chain reaction ; Genotyping ; Czech Republic ; Phylogeography
英文摘要:	Population surveys of Blumeria graminis f. sp. hordei (Bgh), a causal agent of more than 50% of barley fungal infections in the Czech Republic, have been traditionally based on virulence tests, at times supplemented with non-specific Restriction fragment length polymorphism or Random amplified polymorphic DNA markers. A genomic sequence of Bgh, which has become available recently, enables identification of potential markers suitable for population genetics studies. Two major strategies relying on transposable elements and microsatellites were employed in this work to develop a set of Repeat junction markers, Single sequence repeat and Single nucleotide polymorphism markers. A resolution power of the new panel of markers comprising 33 polymorphisms was demonstrated by a phylogenetic analysis of 158 Bgh isolates. A core set of 97 Czech isolates was compared to a set 50 Australian isolates on the background of 11 diverse isolates collected throughout the world. 73.2% of Czech isolates were found to be genetically unique. An extreme diversity of this collection was in strong contrast with the uniformity of the Australian one. This work paves the way for studies of population structure and dynamics based on genetic variability among different Bgh isolates originating from geographically limited regions.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0167099&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/24039
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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File Name/ File Size	Content Type	Version	Access	License
journal.pone.0167099.pdf（2165KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic;Department of Integrated Plant Protection, Agrotest Fyto Ltd., Kroměříž, Czech Republic;Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic;Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic;Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic

Recommended Citation:

Eva Komínková,Antonín Dreiseitl,Eva Malečková,et al. Genetic Diversity of Blumeria graminis f. sp. hordei in Central Europe and Its Comparison with Australian Population[J]. PLOS ONE,2016-01-01,11(11)

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