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DOI:	10.1371/journal.pone.0167583
论文题名:	A Modular and Affordable Time-Lapse Imaging and Incubation System Based on 3D-Printed Parts, a Smartphone, and Off-The-Shelf Electronics
作者:	Rodrigo Hernández Vera; Emil Schwan; Nikos Fatsis-Kavalopoulos; Johan Kreuger
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2016
发表日期:	2016-12-21
卷:	11, 期:12
语种:	英语
英文关键词:	3D printing ; Cell phones ; HEK 293 cells ; Microfluidics ; Cell cultures ; Shutters ; Humidity ; In vivo imaging
英文摘要:	Time-lapse imaging is a powerful tool for studying cellular dynamics and cell behavior over long periods of time to acquire detailed functional information. However, commercially available time-lapse imaging systems are expensive and this has limited a broader implementation of this technique in low-resource environments. Further, the availability of time-lapse imaging systems often present workflow bottlenecks in well-funded institutions. To address these limitations we have designed a modular and affordable time-lapse imaging and incubation system (ATLIS). The ATLIS enables the transformation of simple inverted microscopes into live cell imaging systems using custom-designed 3D-printed parts, a smartphone, and off-the-shelf electronic components. We demonstrate that the ATLIS provides stable environmental conditions to support normal cell behavior during live imaging experiments in both traditional and evaporation-sensitive microfluidic cell culture systems. Thus, the system presented here has the potential to increase the accessibility of time-lapse microscopy of living cells for the wider research community.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0167583&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/25197
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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File Name/ File Size	Content Type	Version	Access	License
journal.pone.0167583.pdf（2326KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden;Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden;Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden;Gradientech AB, Uppsala Science Park, Uppsala, Sweden;Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden

Recommended Citation:

Rodrigo Hernández Vera,Emil Schwan,Nikos Fatsis-Kavalopoulos,et al. A Modular and Affordable Time-Lapse Imaging and Incubation System Based on 3D-Printed Parts, a Smartphone, and Off-The-Shelf Electronics[J]. PLOS ONE,2016-01-01,11(12)

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