全球变化知识资源中心

Knowledge Resources Center for Global Change


Email:
Passwd
验证:
	换一张
Have you forgotten your password? Stay signed in Log In

globalchange > 过去全球变化的重建

DOI:	10.1371/journal.pone.0169345
论文题名:	Signal-Amplified Lateral Flow Test Strip for Visual Detection of Cu2+
作者:	Yulong Wang; Limin Wang; Juanjuan Xue; Jinbo Dong; Jia Cai; Xiude Hua; Minghua Wang; Cunzheng Zhang; Fengquan Liu
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2017
发表日期:	2017-1-10
卷:	12, 期:1
语种:	英语
英文关键词:	Surface water ; Signal amplification ; Vitamin C ; Alkynes ; Azides ; Rivers ; Probe hybridization ; Reaction time
英文摘要:	A signal-amplified lateral flow test strip (SA-LFTS) for the detection of Cu2+ in aqueous solution was constructed based on Cu+-catalyzed click chemistry and hybridization of single-stranded DNA (ssDNA). Alkyne and azide modified ssDNA acted as specific elements for Cu2+ recognition, and a chemical ligation product formed through Cu+-catalyzed alkyne–azide cycloaddition. Hybridization of ssDNA-labeled gold nanoparticles resulted in high sensitivity, and the output signal could be observed directly by the naked eye. Using the developed SA-LFTS under optimal conditions, Cu2+ could be detected rapidly with limit of detections of 5 nM and 4.2 nM by visual observation and quantitative analysis, respectively. The sensitivity (i.e. the visual limit of detection) of the SA-LFTS was 80-times higher than that of traditional LFTS. The SA-LFTS was applied to the determination of Cu2+ in municipal water and river water samples with the results showing good recovery and accuracy. The developed test strip is promising for point-of-care applications and detection of Cu2+ in the field.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0169345&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/25707
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

Files in This Item:

File Name/ File Size	Content Type	Version	Access	License
journal.pone.0169345.pdf（2042KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Institute of Plant Protection, Jiangsu Academy of Agricultural Science, College of Plant Protection, Nanjing Agricultural University, Nanjing, P. R. China;College of Plant Protection (Key Laboratory of Integrated Management of Crop Diseases and Pests), Nanjing Agricultural University, Nanjing, P. R. China;College of Plant Protection (Key Laboratory of Integrated Management of Crop Diseases and Pests), Nanjing Agricultural University, Nanjing, P. R. China;College of Plant Protection (Key Laboratory of Integrated Management of Crop Diseases and Pests), Nanjing Agricultural University, Nanjing, P. R. China;College of Plant Protection (Key Laboratory of Integrated Management of Crop Diseases and Pests), Nanjing Agricultural University, Nanjing, P. R. China;College of Plant Protection (Key Laboratory of Integrated Management of Crop Diseases and Pests), Nanjing Agricultural University, Nanjing, P. R. China;College of Plant Protection (Key Laboratory of Integrated Management of Crop Diseases and Pests), Nanjing Agricultural University, Nanjing, P. R. China;Institute of Food Quality Safety and Detection Research, Jiangsu Academy of Agricultural Sciences, Nanjing, P. R. China;Institute of Plant Protection, Jiangsu Academy of Agricultural Science, College of Plant Protection, Nanjing Agricultural University, Nanjing, P. R. China;College of Plant Protection (Key Laboratory of Integrated Management of Crop Diseases and Pests), Nanjing Agricultural University, Nanjing, P. R. China

Recommended Citation:

Yulong Wang,Limin Wang,Juanjuan Xue,et al. Signal-Amplified Lateral Flow Test Strip for Visual Detection of Cu2+[J]. PLOS ONE,2017-01-01,12(1)

Service
	Recommend this item
	Sava as my favorate item
	Show this item's statistics
	Export Endnote File
Google Scholar
	Similar articles in Google Scholar
	[Yulong Wang]'s Articles
	[Limin Wang]'s Articles
	[Juanjuan Xue]'s Articles
百度学术
	Similar articles in Baidu Scholar
	[Yulong Wang]'s Articles
	[Limin Wang]'s Articles
	[Juanjuan Xue]'s Articles
CSDL cross search
	Similar articles in CSDL Cross Search
	[Yulong Wang]‘s Articles
	[Limin Wang]‘s Articles
	[Juanjuan Xue]‘s Articles
Related Copyright Policies
Null
收藏/分享