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globalchange > 过去全球变化的重建

DOI:	10.1371/journal.pone.0170945
论文题名:	Surveying Europe’s Only Cave-Dwelling Chordate Species (Proteus anguinus) Using Environmental DNA
作者:	Judit Vörös; Orsolya Márton; Benedikt R. Schmidt; Júlia Tünde Gál; Dušan Jelić
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2017
发表日期:	2017-1-27
卷:	12, 期:1
语种:	英语
英文关键词:	Polymerase chain reaction ; Caves ; Precipitation techniques ; Croatia ; Filter sterilization ; DNA sequence analysis ; DNA extraction ; Biodiversity
英文摘要:	In surveillance of subterranean fauna, especially in the case of rare or elusive aquatic species, traditional techniques used for epigean species are often not feasible. We developed a non-invasive survey method based on environmental DNA (eDNA) to detect the presence of the red-listed cave-dwelling amphibian, Proteus anguinus, in the caves of the Dinaric Karst. We tested the method in fifteen caves in Croatia, from which the species was previously recorded or expected to occur. We successfully confirmed the presence of P. anguinus from ten caves and detected the species for the first time in five others. Using a hierarchical occupancy model we compared the availability and detection probability of eDNA of two water sampling methods, filtration and precipitation. The statistical analysis showed that both availability and detection probability depended on the method and estimates for both probabilities were higher using filter samples than for precipitation samples. Combining reliable field and laboratory methods with robust statistical modeling will give the best estimates of species occurrence.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0170945&type=printable
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资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/25820
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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journal.pone.0170945.pdf（1897KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Department of Zoology, Hungarian Natural History Museum, Budapest, Hungary;Molecular Taxonomy Laboratory, Hungarian Natural History Museum, Budapest, Hungary;Institute for Soil Sciences and Agricultural Chemistry, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary;Department of Evolutionary Biology and Environment Studies, University of Zurich, Zurich, Switzerland;Koordinationsstelle für Amphibien- und Reptilienschutz in der Schweiz, Neuchâtel, Switzerland;Department of Zoology, Hungarian Natural History Museum, Budapest, Hungary;Croatian Institute for Biodiversity, Zagreb, Croatia

Recommended Citation:

Judit Vörös,Orsolya Márton,Benedikt R. Schmidt,et al. Surveying Europe’s Only Cave-Dwelling Chordate Species (Proteus anguinus) Using Environmental DNA[J]. PLOS ONE,2017-01-01,12(1)

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