| DOI: | 10.2172/985928
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| 报告号: | LBNL-3734E-Poster
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| 报告题名: | Effects of Nitrate Exposure on the Functional Structure of a Microbial Community in a Uranium-contaminated Aquifer |
| 作者: | Van Nostrand, Joy; Waldron, P.; Wu, W.; Zhou, B.; Wu, Liyou; Deng, Ye; Carley, J.; He, Zhili; Gu, B.; Luo, J.; Criddle, C.; Jardine, P.; Hazen, Terry; Zhou, Jizhong
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| 出版年: | 2010
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| 发表日期: | 2010-05-17
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| 总页数: | 1
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| 国家: | 美国
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| 语种: | 英语
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| 英文关键词: | BioCON
; ecological experiment nitrogen desposition
; microbial community
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| 中文主题词: | 氮
; 硝酸盐
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| 主题词: | NITROGEN
; NITRATE
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| 英文摘要: | Increasing nitrogen deposition, increasing atmospheric CO2, and decreasing biodiversity are three main environmental changes occurring on a global scale. The BioCON (Biodiversity, CO2, and Nitrogen) ecological experiment site at the University of Minnesota's Cedar Creek Ecosystem Science Reserve started in 1997, to better understand how these changes would affect soil systems. To understand how increasing nitrogen deposition affects the microbial community diversity, heterogeneity, and functional structure impact soil microbial communities, 12 samples were collected from the BioCON plots in which nitrogenous fertilizer was added to simulate the effect of increasing nitrogen deposition and 12 samples from without added fertilizer. DNA from the 24 samples was extracted using a freeze-grind protocol, amplified, labeled with a fluorescent dye, and then hybridized to GeoChip, a functional gene array containing probes for genes involved in N, S and C cycling, metal resistance and organic contaminant degradation. Detrended correspondence analysis (DCA) of all genes detected was performed to analyze microbial community patterns. The first two axes accounted for 23.5percent of the total variation. The samples fell into two major groups: fertilized and non-fertilized, suggesting that nitrogenous fertilizer had a significant impact on soil microbial community structure and diversity. The functional gene numbers detected in fertilized samples was less that detected in non-fertilizer samples. Functional genes involving in the N cycling were mainly discussed. |
| URL: | http://www.osti.gov/scitech/servlets/purl/985928
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| Citation statistics: |
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| 资源类型: | 研究报告
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/39802
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| Appears in Collections: | 过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响
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| 985928.pdf（714KB） | 研究报告 | -- | 开放获取 | | View
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| Recommended Citation: | |
Van Nostrand, Joy,Waldron, P.,Wu, W.,et al. Effects of Nitrate Exposure on the Functional Structure of a Microbial Community in a Uranium-contaminated Aquifer. 2010-01-01.
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