| DOI: | 10.2172/1052840
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| 报告号: | DOE-SC0000587-1
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| 报告题名: | Three-Dimensional Imaging and Quantification of Biomass and Biofilms in Porous Media |
| 作者: | Dorthe Wildenschild
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| 出版年: | 2012
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| 发表日期: | 2012-10-10
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| 国家: | 美国
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| 语种: | 英语
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| 中文主题词: | 生物量
; 微量营养物/微量元素
; 密度
; X射线
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| 主题词: | BIOMASS
; NUTRIENTS
; DENSITY
; X-RAY
|
| 英文摘要: | A new method to resolve biofilms in three dimensions in porous media using high-resolution synchrotron-based x-ray computed microtomography (CMT) has been developed. Imaging biofilms in porous media without disturbing the natural spatial arrangement of the porous media and associated biofilm has been a challenging task, primarily because porous media generally precludes conventional imaging via optical microscopy; x-ray tomography offers a potential alternative. One challenge for using this method is that most conventional x-ray contrast agents are water-soluble and easily diffuse into biofilms. To overcome this problem, silver-coated microspheres were added to the fluid phase to create an x-ray contrast that does not diffuse into the biofilm mass. Using this approach, biofilm imaging in porous media was accomplished with sufficient contrast to differentiate between the biomass- and fluid-filled pore spaces. The method was validated by using a two-dimensional micro-model flow cell where both light microscopy and CMT imaging were used to im age the biofilm. The results of this work has been published in Water Resources Research (Iltis et al., 2010). Additional work needs to be done to optimize this imaging approach, specifically, we find that the quality of the images are highly dependent on the coverage of the biofilm with Ag particles, - which means that we may have issues in dead-end pore space and for very low density (fluffy) biofilms. What we can image for certain with this technique is the biofilm surface that is well-connected to flow paths and thus well-supplied with nutrients etc. |
| URL: | http://www.osti.gov/scitech/servlets/purl/1052840
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| Citation statistics: |
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| 资源类型: | 研究报告
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/40495
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| Appears in Collections: | 过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响
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| 1052840.pdf（482KB） | 研究报告 | -- | 开放获取 | | View
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| Recommended Citation: | |
Dorthe Wildenschild. Three-Dimensional Imaging and Quantification of Biomass and Biofilms in Porous Media. 2012-01-01.
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