| DOI: | 10.2172/1043690
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| 报告号: | DOE/ER/63427-2 Final Report
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| 报告题名: | Defining How a Microbial Cell Senses and Responds to a Redox Active Environment |
| 作者: | Kenneth H. Nealson
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| 出版年: | 2012
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| 发表日期: | 2012-06-22
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| 国家: | 美国
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| 语种: | 英语
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| 英文关键词: | Shawanella
; electron transport
; environmental microbiology
; metal reduction
; microbial genomics
|
| 中文主题词: | 吸收
; 吸附作用
; 铁
; X射线
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| 主题词: | ABSORPTION
; IRON
; X-RAY
|
| 英文摘要: | This grant was for four years, and the work was designed to look at the mechanisms of extracellular electron transfer by the dissimilatory iron reducing bacteria Shewanella oneidensis MR-1, and other closely related Shewanella strains and species. During this work, we defined many of the basic physiological and biochemical properties of the Shewanella group, Much of which was summarized in review articles. We also finished and published the genome sequence of strain MR-1, the first of the shewanellae to have its genome sequenced. Control at the transcriptional and translational level was studied in collaboration with colleagues at PNNL and ANL. We utilized synchrotron X-ray radiation to image both the bacteria and the metal oxide particles via a technique called STXM, synchrotron X-ray absorption (ref. No.9), and X-ray microbeam analysis. We purified several of the cytochromes involved with metal reduction, and improved gene annotation of the MR-1 genome. The conductive appendages (nanowires) of MR-1 were described and characterized. Comparative genomics and biochemistry revealed that the pathway for the utilization of N-acetyl glucosamine in the various strains of Shewanella exhibited great variability, and had a number of previously unknown genes. |
| URL: | http://www.osti.gov/scitech/servlets/purl/1043690
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| Citation statistics: |
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| 资源类型: | 研究报告
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/40626
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| Appears in Collections: | 过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响
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| 1043690.pdf（73KB） | 研究报告 | -- | 开放获取 | | View
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| Recommended Citation: | |
Kenneth H. Nealson. Defining How a Microbial Cell Senses and Responds to a Redox Active Environment. 2012-01-01.
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