| DOI: | 10.2172/1051498
|
| 报告号: | None
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| 报告题名: | Characterization of Methane Degradation and Methane-Degrading Microbes in Alaska Coastal Water |
| 作者: | Kirchman, David L. [Univ. of Delaware, Lewes, DE (United States)]
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| 出版年: | 2012
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| 发表日期: | 2012-03-29
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| 总页数: | 23
|
| 国家: | 美国
|
| 语种: | 英语
|
| 中文主题词: | 大气科学
; 碳
; 剥蚀
; 沉积物
; 有机碳
; 甲烷
|
| 主题词: | ATMOSPHERE
; CARBON
; DEGRADATION
; SEDIMENTS
; ORGANIC CARBON
; METHANE
|
| 英文摘要: | The net flux of methane from methane hydrates and other sources to the atmosphere depends on methane degradation as well as methane production and release from geological sources. The goal of this project was to examine methane-degrading archaea and organic carbon oxidizing bacteria in methane-rich and methane-poor sediments of the Beaufort Sea, Alaska. The Beaufort Sea system was sampled as part of a multi-disciplinary expedition (Methane in the Arctic Shelf or MIDAS) in September 2009. Microbial communities were examined by quantitative PCR analyses of 16S rRNA genes and key methane degradation genes (pmoA and mcrA involved in aerobic and anaerobic methane degradation, respectively), tag pyrosequencing of 16S rRNA genes to determine the taxonomic make up of microbes in these sediments, and sequencing of all microbial genes (metagenomes ). The taxonomic and functional make-up of the microbial communities varied with methane concentrations, with some data suggesting higher abundances of potential methane-oxidizing archaea in methane-rich sediments. Sequence analysis of PCR amplicons revealed that most of the mcrA genes were from the ANME-2 group of methane oxidizers. According to metagenomic data, genes involved in methane degradation and other degradation pathways changed with sediment depth along with sulfate and methane concentrations. Most importantly, sulfate reduction genes decreased with depth while the anaerobic methane degradation gene (mcrA) increased along with methane concentrations. The number of potential methane degradation genes (mcrA) was low and inconsistent with other data indicating the large impact of methane on these sediments. The data can be reconciled if a small number of potential methane-oxidizing archaea mediates a large flux of carbon in these sediments. Our study is the first to report metagenomic data from sediments dominated by ANME-2 archaea and is one of the few to examine the entire microbial assemblage potentially involved in anaerobic methane oxidation. |
| URL: | http://www.osti.gov/scitech/servlets/purl/1051498
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| Citation statistics: |
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| 资源类型: | 研究报告
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/40716
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| Appears in Collections: | 过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响
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Version |
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| 1051498.pdf（477KB） | 研究报告 | -- | 开放获取 | | View
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| Recommended Citation: | |
Kirchman, David L. [Univ. of Delaware, Lewes, DE . Characterization of Methane Degradation and Methane-Degrading Microbes in Alaska Coastal Water. 2012-01-01.
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