| DOI: | 10.2172/1077983
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| 报告号: | DOE/ER--63442
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| 报告题名: | Heritable Genetic Changes in Cells Recovered From Irradiated 3D Tissue Contracts. Final report |
| 作者: | Cornforth, Michael N. [The University of Texas Medical Branch at Galveston, TX (United States)]
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| 出版年: | 2013
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| 发表日期: | 2013-05-03
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| 总页数: | 9
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| 国家: | 美国
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| 语种: | 英语
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| 中文主题词: | 荧光
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| 主题词: | FLUORESCENCE
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| 英文摘要: | Combining contemporary cytogenetic methods with DNA CGH microarray technology and chromosome flow-sorting increases substantially the ability to resolve exchange breakpoints associated with interstitial deletions and translocations, allowing the consequences of radiation damage to be directly measured at low doses, while also providing valuable insights into molecular mechanisms of misrepair processes that, in turn, identify appropriate biophysical models of risk at low doses. The aims of this work apply to cells recovered from 3D tissue constructs of human skin and, for the purpose of comparison, the same cells irradiated in traditional 2D cultures. These aims are: to analyze by multi-flour fluorescence in situ hybridization (mFISH) the chromosomes in clonal descendents of individual human fibroblasts that were previously irradiated; to examine irradiated clones from Aim 1 for submicroscopic deletions by subjecting their DNA to comparative genomic hybridization (CGH) microarray analysis; and to flow-sort aberrant chromosomes from clones containing stable radiation-induced translocations and map the breakpoints to within an average resolution of 100 kb using the technique of 'array painting'. |
| URL: | http://www.osti.gov/scitech/servlets/purl/1077983
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| Citation statistics: |
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| 资源类型: | 研究报告
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/41149
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| Appears in Collections: | 过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响
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| 1077983.pdf（756KB） | 研究报告 | -- | 开放获取 | | View
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| Recommended Citation: | |
Cornforth, Michael N. [The University of Texas Medical Branch at Galveston, TX . Heritable Genetic Changes in Cells Recovered From Irradiated 3D Tissue Contracts. Final report. 2013-01-01.
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