| DOI: | 10.2172/1167142
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| 报告号: | DOE-UTK--0004796
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| 报告题名: | MURMoT. Design and Application of Microbial Uranium Reduction Monitoring Tools |
| 作者: | Loeffler, Frank E.
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| 出版年: | 2014
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| 发表日期: | 2014-12-31
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| 总页数: | 10
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| 国家: | 美国
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| 语种: | 英语
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| 英文关键词: | Uranium
; microbial activity
; immobilization
; monitoring tools
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| 英文摘要: | Uranium (U) contamination in the subsurface is a major remediation challenge at many DOE sites. Traditional site remedies present enormous costs to DOE; hence, enhanced bioremediation technologies (i.e., biostimulation and bioaugmentation) combined with monitoring efforts are being considered as cost-effective corrective actions to address subsurface contamination. This research effort improved understanding of the microbial U reduction process and developed new tools for monitoring microbial activities. Application of these tools will promote science-based site management decisions that achieve contaminant detoxification, plume control, and long-term stewardship in the most efficient manner. The overarching hypothesis was that the design, validation and application of a suite of new molecular and biogeochemical tools advance process understanding, and improve environmental monitoring regimes to assess and predict in situ U immobilization. Accomplishments: This project (i) advanced nucleic acid-based approaches to elucidate the presence, abundance, dynamics, spatial distribution, and activity of metal- and radionuclide-detoxifying bacteria; (ii) developed proteomics workflows for detection of metal reduction biomarker proteins in laboratory cultures and contaminated site groundwater; (iii) developed and demonstrated the utility of U isotopic fractionation using high precision mass spectrometry to quantify U(VI) reduction for a range of reduction mechanisms and environmental conditions; and (iv) validated the new tools using field samples from U-contaminated IFRC sites, and demonstrated their prognostic and diagnostic capabilities in guiding decision making for environmental remediation and long-term site stewardship. |
| URL: | http://www.osti.gov/scitech/servlets/purl/1167142
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| Citation statistics: |
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| 资源类型: | 研究报告
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/41316
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| Appears in Collections: | 过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响
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| 1167142.pdf（330KB） | 研究报告 | -- | 开放获取 | | View
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作者单位: | Univ. of Tennessee, Knoxville, TN (United States)
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| Recommended Citation: | |
Loeffler, Frank E.. MURMoT. Design and Application of Microbial Uranium Reduction Monitoring Tools. 2014-01-01.
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