DOI: | 10.2172/1164471
|
报告号: | DOE-CALTECH-04949
|
报告题名: | Syntrophic interactions and mechanisms underpinning anaerobic methane oxidation: targeted metaproteogenomics, single-cell protein detection and quantitative isotope imaging of microbial consortia |
作者: | Orphan, Victoria Jeanne
|
出版年: | 2014
|
发表日期: | 2014-11-26
|
国家: | 美国
|
语种: | 英语
|
英文关键词: | methane
; syntrophy
; stable isotope probing
; nanoSIMS
; proteomics
|
中文主题词: | 碳
; 铅
; 甲烷
|
主题词: | CARBON
; LEAD
; METHANE
|
英文摘要: | Syntrophy and mutualism play a central role in carbon and nutrient cycling by microorganisms. Yet, our ability to effectively study symbionts in culture has been hindered by the inherent interdependence of syntrophic associations, their dynamic behavior, and their frequent existence at thermodynamic limits. Now solutions to these challenges are emerging in the form of new methodologies. Developing strategies that establish links between the identity of microorganisms and their metabolic potential, as well as techniques that can probe metabolic networks on a scale that captures individual molecule exchange and processing, is at the forefront of microbial ecology. Understanding the interactions between microorganisms on this level, at a resolution previously intractable, will lead to our greater understanding of carbon turnover and microbial community resilience to environmental perturbations. In this project, we studied an enigmatic syntrophic association between uncultured methane-oxidizing archaea and sulfate-reducing bacteria. This environmental archaeal-bacterial partnership represents a globally important sink for methane in anoxic environments. The specific goals of this project were organized into 3 major tasks designed to address questions relating to the ecophysiology of these syntrophic organisms under changing environmental conditions (e.g. different electron acceptors and nutrients), primarily through the development of microanalytical imaging methods which enable the visualization of the spatial distribution of the partners within aggregates, consumption and exchange of isotopically labeled substrates, and expression of targeted proteins identified via metaproteomics. The advanced tool set developed here to collect, correlate, and analyze these high resolution image and isotope-based datasets from methane-oxidizing consortia has the potential to be widely applicable for studying and modeling patterns of activity and interactions across a broad range of spatially structured microbial partnerships, including other syntrophic associations, microbial mats, biofilms, and plant-microbe or animal-microbe symbioses in nature. |
URL: | http://www.osti.gov/scitech/servlets/purl/1164471
|
Citation statistics: |
|
资源类型: | 研究报告
|
标识符: | http://119.78.100.158/handle/2HF3EXSE/41345
|
Appears in Collections: | 过去全球变化的重建 影响、适应和脆弱性 科学计划与规划 气候变化与战略 全球变化的国际研究计划 气候减缓与适应 气候变化事实与影响
|
File Name/ File Size |
Content Type |
Version |
Access |
License |
|
1164471.pdf(1433KB) | 研究报告 | -- | 开放获取 | | View
Download
|
|
作者单位: | California Institute of Technology
|
Recommended Citation: |
Orphan, Victoria Jeanne. Syntrophic interactions and mechanisms underpinning anaerobic methane oxidation: targeted metaproteogenomics, single-cell protein detection and quantitative isotope imaging of microbial consortia. 2014-01-01.
|
|
|