| DOI: | 10.2172/1234698
|
| 报告号: | DOE/ER-64787--1033233-0015382
|
| 报告题名: | Low Dose Ionizing Radiation Modulates Immune Function |
| 作者: | Nelson, Gregory A.
|
| 出版年: | 2016
|
| 发表日期: | 2016-01-12
|
| 总页数: | 36
|
| 国家: | 美国
|
| 语种: | 英语
|
| 英文关键词: | Low dose immune function
|
| 中文主题词: | 冰上通道
; 水道
|
| 主题词: | LEADS
|
| 英文摘要: | In order to examine the effects of low dose ionizing radiation on the immune system we chose to examine an amplified adaptive cellular immunity response. This response is Type IV delayed-type hypersensitivity also called contact hypersensitivity. The agent fluorescein isothiocyanate (FITC) is a low molecular weight, lipophilic, reactive, fluorescent molecule that can be applied to the skin where it (hapten) reacts with proteins (carriers) to become a complete antigen. Exposure to FITC leads to sensitization which is easily measured as a hypersensitivity inflammatory reaction following a subsequent exposure to the ear. Ear swelling, eosinophil infiltration, immunoglobulin E production and cytokine secretion patterns characteristic of a âTh2 polarizedâ immune response are the components of the reaction. The reaction requires successful implementation of antigen processing and presentation by antigen presenting Langerhans cells, communication with naĂŻve T lymphocytes in draining lymph nodes, expansion of activated T cell clones, migration of activated T cells to the circulation, and recruitment of memory T cells, macrophages and eosinophils to the site of the secondary challenge. Using this model our approach was to quantify system function rather than relying only on indirect biomarkers of cell. We measured the FITC-induced hypersensitivity reaction over a range of doses from 2 cGy to 2 Gy. Irradiations were performed during key events or prior to key events to deplete critical cell populations. In addition to quantifying the final inflammatory response, we assessed cell populations in peripheral blood and spleen, cytokine signatures, IgE levels and expression of genes associated with key processes in sensitization and elicitation/recall. We hypothesized that ionizing radiation would produce a biphasic effect on immune system function resulting in an enhancement at low doses and a depression at higher doses and suggested that this transition would occur in the dose range of 5 to 50 cGy. |
| URL: | http://www.osti.gov/scitech/servlets/purl/1234698
|
| Citation statistics: |
|
| 资源类型: | 研究报告
|
| 标识符: | http://119.78.100.158/handle/2HF3EXSE/42302
|
| Appears in Collections: | 过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响
|
| File Name/ File Size |
Content Type |
Version |
Access |
License |
|
|---|
| 1234698.pdf（4802KB） | 研究报告 | -- | 开放获取 | | View
Download
|
|
| Recommended Citation: | |
Nelson, Gregory A.. Low Dose Ionizing Radiation Modulates Immune Function. 2016-01-01.
|
|
|