DOI: 10.1016/j.jcou.2016.11.011
Scopus记录号: 2-s2.0-85006355975
论文题名: Supercritical carbon dioxide as solvent in the lipase-catalyzed ethanolysis of fish oil: Kinetic study
作者: Melgosa R. ; Teresa Sanz M. ; Solaesa A.G. ; De Paz E. ; Beltrán S. ; Lamas D.L.
刊名: Journal of CO2 Utilization
ISSN: 22129820
出版年: 2017
卷: 17 起始页码: 170
结束页码: 179
语种: 英语
英文关键词: Ethanolysis
; Lipase
; Omega 3
; Supercritical carbon dioxide
Scopus关键词: Activation energy
; Catalysis
; Enzyme inhibition
; Enzymes
; Ethanol
; Fish
; Gases
; Kinetic theory
; Kinetics
; Lipases
; Rate constants
; Reaction rates
; Solvents
; Supercritical fluid extraction
; Equilibrium conversion
; Ethanol/oil molar ratios
; Ethanolysis
; Ethanolysis reactions
; Experimental conditions
; Kinetic rate constants
; Omega-3
; Supercritical carbon dioxides
; Carbon dioxide
英文摘要: Supercritical carbon dioxide (SC-CO2) has been used as green solvent in the lipase-catalyzed ethanolysis of fish oil by Lipozyme RM IM at mild, non-oxidative conditions and with no solvent residues. The effect of experimental conditions, initial substrate ethanol/oil molar ratio (2-38), pressure (7.5-30 MPa), and temperature (323.15-353.15 K) on equilibrium conversion, reaction rate and oxidative status of the products has been studied. No ethanol inhibition has been observed at high concentrations of ethanol, when putting in contact first the fish oil with the enzyme avoiding direct contact between the biocatalyst and ethanol. Operating pressure affected positively the reaction performance in the range investigated. Visual observation of the phase behaviour of the initial reaction mixture showed an "expanded liquid phase" that helped enhancing reaction rate, and a gas phase. Raising temperature accelerated the reaction up to a limit (343.15 K), observing higher enzyme thermal stability than in other reaction media (313.15 K). However, lipid oxidation increases with temperature. Up to 86 ± 1% FAEE yield has been found at MR = 6:1, 30 MPa and 323.15 K. Kinetic data have been correlated by using a mathematical model based on the elementary reactions of the 3-step transesterification. Kinetic rate constants, apparent activation volumes and energies are reported for the first time for a lipase-catalyzed ethanolysis reaction in SC-CO2. © 2016 Elsevier Ltd. All rights reserved. 资助项目: European Regional Development Fund
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/52800
Appears in Collections: 影响、适应和脆弱性
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Recommended Citation:
Melgosa R.,Teresa Sanz M.,Solaesa A.G.,et al. Supercritical carbon dioxide as solvent in the lipase-catalyzed ethanolysis of fish oil: Kinetic study[J]. Journal of CO2 Utilization,2017-01-01,17