globalchange  > 影响、适应和脆弱性
DOI: 10.1111/gcb.13249
论文题名:
Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors
作者: Dineshram R.; Chandramouli K.; Ko G.W.K.; Zhang H.; Qian P.-Y.; Ravasi T.; Thiyagarajan V.
刊名: Global Change Biology
ISSN: 13541013
出版年: 2016
卷: 22, 期:6
起始页码: 2054
结束页码: 2068
语种: 英语
英文关键词: iTRAQ ; Climate change ; Crassostrea gigas ; Environmental proteomics ; Multiple stressors ; Ocean acidification
Scopus关键词: acidification ; climate change ; climate effect ; environmental stress ; larva ; metabolism ; metamorphosis ; mollusc ; physiological response ; protein ; quantitative analysis ; survival ; Crassostrea gigas ; Ostreidae ; proteome ; sea water ; adaptation ; animal ; chemistry ; climate change ; Crassostrea ; larva ; liquid chromatography ; metamorphosis ; pH ; physiological stress ; physiology ; salinity ; tandem mass spectrometry ; temperature ; Adaptation, Physiological ; Animals ; Chromatography, Liquid ; Climate Change ; Crassostrea ; Hydrogen-Ion Concentration ; Larva ; Metamorphosis, Biological ; Proteome ; Salinity ; Seawater ; Stress, Physiological ; Tandem Mass Spectrometry ; Temperature
英文摘要: The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ-LC-MS/MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down-regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down-regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up-regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs. © 2016 John Wiley & Sons Ltd.
资助项目: Acknowledgements The authors would like to thank Ziniu Yu and Yang Zhang of South China Sea Institute of Oceanology for providing the oysters used in our experiments and for helping us with RT-PCR analysis. We thank Ivan Chiu (University of Hong Kong), and Yun Lam (City University of Hong Kong) for technical support and for providing access to sample preparation and data analysis. The collaboration between VT and PYQ is supported by the State Key Laboratory in Marine Pollution of City University of Hong Kong. We thank Gabriela Bindea of Cordeliers Research Center for adding Crassostrea gigas annotation resources in ClueGO for pathway analysis. We thank P. Sriyutha Murthy (BARC, Kalpakkam, India) for proofreading. We are also grateful to the three anonymous reviewers and the editor for their substantial contributions to this article. This study was funded by three GRF grants from the HKSAR-RGC (Grant Numbers: 705112P, 17304914, and 705511P).
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/61390
Appears in Collections:影响、适应和脆弱性

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作者单位: The Swire Institute of Marine Science and School of Biological Sciences, The University of Hong Kong, Pok Fu Lam Road, Hong Kong Island, Hong Kong; Division of Biological, King Abdullah University of Science and Technology, Environmental Sciences and Engineering, Division of Applied Mathematics and Computer Sci. and KAUST Environmental Epigenetic Program (KEEP), Thuwal, Saudi Arabia; Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong

Recommended Citation:
Dineshram R.,Chandramouli K.,Ko G.W.K.,et al. Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors[J]. Global Change Biology,2016-01-01,22(6)
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