DOI: 10.1111/gcb.12618
论文题名: Nitrogen and phosphorus additions impact arbuscular mycorrhizal abundance and molecular diversity in a tropical montane forest
作者: Camenzind T. ; Hempel S. ; Homeier J. ; Horn S. ; Velescu A. ; Wilcke W. ; Rillig M.C.
刊名: Global Change Biology
ISSN: 13541013
出版年: 2014
卷: 20, 期: 12 起始页码: 3646
结束页码: 3659
语种: 英语
英文关键词: NUMEX
; Arbuscular mycorrhizal fungi
; Biodiversity
; Community
; Ecuador
; Fertilization
; Pyrosequencing
; Tropical montane forest
Scopus关键词: Fungi
; nitrogen
; phosphorus
; ribosome RNA
; soil
; anatomy and histology
; biodiversity
; biological model
; chemistry
; climate change
; DNA sequence
; Ecuador
; forecasting
; genetics
; growth, development and aging
; microbiology
; molecular genetics
; mycorrhiza
; nucleotide sequence
; plant root
; procedures
; soil
; species difference
; Base Sequence
; Biodiversity
; Climate Change
; Ecuador
; Forecasting
; Models, Biological
; Molecular Sequence Data
; Mycorrhizae
; Nitrogen
; Phosphorus
; Plant Roots
; RNA, Ribosomal
; Sequence Analysis, DNA
; Soil
; Species Specificity
英文摘要: Increased nitrogen (N) depositions expected in the future endanger the diversity and stability of ecosystems primarily limited by N, but also often co-limited by other nutrients like phosphorus (P). In this context a nutrient manipulation experiment (NUMEX) was set up in a tropical montane rainforest in southern Ecuador, an area identified as biodiversity hotspot. We examined impacts of elevated N and P availability on arbuscular mycorrhizal fungi (AMF), a group of obligate biotrophic plant symbionts with an important role in soil nutrient cycles. We tested the hypothesis that increased nutrient availability will reduce AMF abundance, reduce species richness and shift the AMF community toward lineages previously shown to be favored by fertilized conditions. NUMEX was designed as a full factorial randomized block design. Soil cores were taken after 2 years of nutrient additions in plots located at 2000 m above sea level. Roots were extracted and intraradical AMF abundance determined microscopically; the AMF community was analyzed by 454-pyrosequencing targeting the large subunit rDNA. We identified 74 operational taxonomic units (OTUs) with a large proportion of Diversisporales. N additions provoked a significant decrease in intraradical abundance, whereas AMF richness was reduced significantly by N and P additions, with the strongest effect in the combined treatment (39% fewer OTUs), mainly influencing rare species. We identified a differential effect on phylogenetic groups, with Diversisporales richness mainly reduced by N additions in contrast to Glomerales highly significantly affected solely by P. Regarding AMF community structure, we observed a compositional shift when analyzing presence/absence data following P additions. In conclusion, N and P additions in this ecosystem affect AMF abundance, but especially AMF species richness; these changes might influence plant community composition and productivity and by that various ecosystem processes. © 2014 John Wiley & Sons Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/62104
Appears in Collections: 影响、适应和脆弱性
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作者单位: Institute of Biology, Freie Universität Berlin, Altensteinstr. 6, Berlin, Germany; Berlin-Brandenburg Institute of Advanced Biodiversity Research (BBIB), Berlin, Germany; Albrecht von Haller Institute of Plant Sciences, University of Göttingen, Untere Karspüle 2, Göttingen, Germany; Geographic Institute, University of Bern, Hallerstr. 12, Bern, Switzerland; Institute of Geography and Geoecology, Karlsruhe Institute of Technology, Kaiserstr. 12, Karlsruhe, Germany
Recommended Citation:
Camenzind T.,Hempel S.,Homeier J.,et al. Nitrogen and phosphorus additions impact arbuscular mycorrhizal abundance and molecular diversity in a tropical montane forest[J]. Global Change Biology,2014-01-01,20(12)