DOI: 10.1016/j.foreco.2017.07.046
Scopus记录号: 2-s2.0-85026857836
论文题名: Quantitative interactions between the biocontrol fungus Phlebiopsis gigantea, the forest pathogen Heterobasidion annosum and the fungal community inhabiting Norway spruce stumps
作者: Oliva J. ; Messal M. ; Wendt L. ; Elfstrand M.
刊名: Forest Ecology and Management
ISSN: 0378-1127
出版年: 2017
卷: 402 起始页码: 253
结束页码: 264
语种: 英语
英文关键词: Butt rot
; Heartwood
; Necrotroph
; Root rot
; Sapwood
; Wood-inhabiting fungi
英文摘要: Protection against the root rot fungus Heterobasidion annosum s. l. is achieved by mechanically spraying stumps with spores of the antagonistic fungus Phlebiopsis gigantea, commercialized as Rotstop®. An extensive colonisation of the stump by P. gigantea is required for protection. Colonisation may depend on how the biocontrol agent interacts with the fungal community that is endophytically inhabiting the stump, as well as with other fungi establishing after the treatment is applied. In this research, we studied how the distribution and abundance of Heterobasidion and P. gigantea is modulated by the fungal community inhabiting Norway spruce (Picea abies) stumps. First thinning stumps were treated with Rotstop and/or artificially inoculated with H. parviporum under field conditions. After two months, P. gigantea or Heterobasidion distribution was measured by qPCR, and both pathogen and biocontrol abundance gradients were correlated with members of the fungal community determined by high throughput sequencing. Rotstop application decreased Heterobasidion biomass by almost 200 times, though the strongest biomass reductions were only achieved when stumps were well covered by P. gigantea. Poor Rotstop applications resulting in, for instance, a drop of 20% in terms of stump covered area by the biocontrol, were associated with a 2-fold biomass increase of the surviving Heterobasidion colonies. Unlike Heterobasidion, some fungi such as Peniophora sp. and F. pinicola did not change their relative abundance following the Rotstop treatment and were associated with areas of low P. gigantea presence. Yeast-like fungi of the genus Debaryomyces increased its relative abundance following Rotstop treatment, and were the only taxa associated with both a higher abundance of the biocontrol, and a lower abundance of the pathogen. Ours is the first study quantifying at microscale the interaction between H. parviporum and P. gigantea in stumps subjected to Rotstop treatment, showing how incomplete stump cover may result in large shifts in terms of biomass for either the pathogen or the biocontrol. Our results stress the importance of achieving a complete cover of the stump surface in practice. Debaryomyces sp. might facilitate P. gigantea colonisation of the stump and could be added as enhancers of Rotstop treatment, though the mechanism of interaction with the pathogen and the biocontrol should be further studied. © 2017 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/64154
Appears in Collections: 影响、适应和脆弱性
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作者单位: Dept. Crop and Forest Sciences, University of Lleida, Alcalde Rovira Roure 191, Lleida, Spain; Dept. of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Box 7026, Uppsala, Sweden; Department of Genetics, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Ring Rd, Hillcrest, Pretoria, South Africa; Helmholtz Centre for Infection Research, Inhoffenstraβe 7, Braunschweig, Germany
Recommended Citation:
Oliva J.,Messal M.,Wendt L.,et al. Quantitative interactions between the biocontrol fungus Phlebiopsis gigantea, the forest pathogen Heterobasidion annosum and the fungal community inhabiting Norway spruce stumps[J]. Forest Ecology and Management,2017-01-01,402