DOI: 10.1016/j.foreco.2017.02.033
Scopus记录号: 2-s2.0-85013772524
论文题名: Physico-chemical and microbiological evidence of exposure effects on Picea abies – Coarse woody debris at different stages of decay
作者: Gómez-Brandón M. ; Ascher-Jenull J. ; Bardelli T. ; Fornasier F. ; Fravolini G. ; Arfaioli P. ; Ceccherini M.T. ; Pietramellara G. ; Lamorski K. ; Sławiński C. ; Bertoldi D. ; Egli M. ; Cherubini P. ; Insam H.
刊名: Forest Ecology and Management
ISSN: 0378-1127
出版年: 2017
卷: 391 起始页码: 376
结束页码: 389
语种: 英语
英文关键词: Climosequence
; Extracellular DNA
; Hydrolytic enzymes
; Quantitative PCR
; Wood decay
; X-ray microtomography
Scopus关键词: Cooling systems
; Damage detection
; Debris
; DNA
; Ecosystems
; Enzyme activity
; Enzymes
; Forestry
; Nutrients
; Plants (botany)
; Polymerase chain reaction
; Tomography
; Wood
; Climosequence
; Extracellular
; Hydrolytic enzyme
; Quantitative PCR
; X ray microtomography
; Decay (organic)
; alpine environment
; bacterium
; coarse woody debris
; coniferous tree
; decomposition
; DNA
; enzyme activity
; fungus
; genetic analysis
; microbial activity
; microbial community
; physicochemical property
; polymerase chain reaction
; tomography
; Decay
; Enzymes
; Nucleic Acids
; Wood
; Italy
; Archaea
; Bacteria (microorganisms)
; Picea abies
英文摘要: Although slope aspect determines the amount of solar irradiation, with implications on the functioning of forest ecosystems, little is known yet about how this affects the aboveground deadwood decomposition dynamics. Therefore, we set up a climosequence case study to evaluate the impact of slope exposure (north- vs. south-facing sites) on the physico-chemical and microbiological properties of Picea abies coarse woody debris (CWD) at different stages of natural decay (decay classes, DCls 1–5) in an Italian Alpine setting. Variations in bacterial, fungal and archaeal abundances were assessed by real-time PCR in the extra- and intracellular DNA fractions (eDNA vs. iDNA) of the total deadwood DNA pool. Physico-chemical wood properties (macro- and micronutrients; lignin and cellulose content; 3D structure via X-ray microtomography) were also performed along with the determination of key enzymatic activities involved in the main nutrient cycles. Overall, higher microbial abundances were registered in Picea abies CWD samples at the cooler, more acidic and moister north-facing site, which are favourable conditions especially for fungal wood decomposers. This thermal signal (N > S) was more evident for the advanced decay stages (DCls 4 and 5), being wood pH the most determinant factor for discriminating between both slopes. We also found that the impact of exposure was enzyme-specific and strongly dependent on the decay class, except for those enzymes involved in the P cycle. In addition, the eDNA/iDNA ratio provided a simple yet powerful index of microbial activity in terms of exposure, with lower values at the north-facing slope indicative of a higher microbial activity. This is in line with the more pronounced physical wood damage detected at this slope by the X-ray microtomography. A higher microbial activity at the cooler north-facing site rather seems surprising – a circumstance that probably is not due to temperature itself but due to increased moisture availability at this slope. © 2017 Elsevier B.V. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/64419
Appears in Collections: 影响、适应和脆弱性
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作者单位: Institute of Microbiology, University of Innsbruck, Technikerstraβe 25d, Innsbruck, Austria; Department of Agrifood and Environmental Science, University of Florence, Piazzale delle Cascine 18, Florence, Italy; Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per lo Studio delle Relazioni tra Pianta e Suolo (C.R.A.-R.P.S.), Via Trieste 23, Gorizia, Italy; Department of Bioscience and Territory, University of Molise, Pesche, Italy; Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, Lublin, Poland; IASMA, Fondazione Edmund Mach, San Michele all'Adige, Italy; Department of Geography, University of Zürich, Winterthurerstrasse 190, Zürich, Switzerland; WSL Swiss Federal Institute for Forest, Snow and Landscape Research, 8903 Birmensdorf, Switzerland
Recommended Citation:
Gómez-Brandón M.,Ascher-Jenull J.,Bardelli T.,et al. Physico-chemical and microbiological evidence of exposure effects on Picea abies – Coarse woody debris at different stages of decay[J]. Forest Ecology and Management,2017-01-01,391