DOI: 10.1016/j.foreco.2016.11.043
Scopus记录号: 2-s2.0-85000360981
论文题名: Realized pollen flow and wildling establishment from a genetically modified eucalypt field trial in Southeastern Brazil
作者: Silva P.H.M.D. ; Sebbenn A.M. ; Grattapaglia D. ; Conti J.L.F. ; Jr.
刊名: Forest Ecology and Management
ISSN: 0378-1127
出版年: 2017
卷: 385 起始页码: 161
结束页码: 166
语种: 英语
英文关键词: Buffer zones
; Genetically modified (GM) tree
; GM pollen flow
; GM wildling establishment
Scopus关键词: Environmental impact
; Plants (botany)
; Polymerase chain reaction
; Reforestation
; Buffer zones
; Experimental assessment
; Genetically modified
; GM wildling establishment
; Negative exponential distribution
; Pollen flows
; Seedling establishment
; Seedling regeneration
; Forestry
; buffer zone
; cultivar
; environmental impact
; evergreen tree
; field method
; gene flow
; genetically modified organism
; grass
; pollen
; pollination
; seed dispersal
; seedling
; seedling establishment
; transgenic plant
; tropical environment
; Brazil
英文摘要: Confined field trials of genetically modified (GM) trees are the essential step toward the identification of the most productive cultivars and the assessment of the likely environmental impacts of the GM trees including the potential for gene flow by pollen and distance dispersal by seeds. Our study investigated the potential for wildling establishment and realized pollen flow from a clonal GM eucalypt field trial in Southeastern Brazil. The GM eucalypt stand was established in 2009, surrounded by a 3 m wide forest road and signal grass (Bracharia sp.) fields. No seedling regeneration was found between 2010 and 2014 in and around the stand, confirming the expectations of the unlikelihood of eucalypt seedling establishment based on its limited invasive potential in competitive tropical environments. In 2014, open-pollinated seeds were collected from 28 non-GM eucalypts located between three and 650 m distance from the GM trial. A total of 420 seedlings were grown in a greenhouse and screened for the presence of the transgenic construct by a multiplexed PCR assay targeting two transgenes and an internal control. The highest average transgene pollen flow (16%) was seen at short distances (3–15 m), rapidly diminishing to 3% by a 240 m distance and continued at this low rate up to the furthest distance assessed (650 m) from the GM trial. The negative exponential distribution of GM pollination success was similar to that observed in non-GM eucalypt pollen flow studies, indicating that low levels of effective pollination are expected over long distances. To the best of our knowledge, this is the first experimental assessments of realized pollen flow measured by the effective production of seedlings from a genetically modified tree in field conditions. © 2016 Elsevier B.V. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/64551
Appears in Collections: 影响、适应和脆弱性
There are no files associated with this item.
作者单位: Instituto de Pesquisas e Estudos Florestais (IPEF), Avenida Pádua Dias 11, Caixa Postal 530, Piracicaba, SP, Brazil; Graduate Program in Forest Sciences FCA/UNESP, Botucatu, SP, Brazil; Instituto Florestal de São Paulo, CP 1322, São Paulo, SP, Brazil; Faculdade de Engenharia de Ilha Solteira/UNESP, Caixa Postal, 31, Ilha Solteira, SP, Brazil; Plant Genetics Laboratory, EMBRAPA Genetic Resources and Biotechnology, Brasilia, DF, Brazil; Graduate Program in Genomic Sciences Biotechnology and Universidade Católica de Brasília, SGAN Qd 916, Brasília, DF, Brazil; ArborGen Tecnologia Florestal, Rua Dr. Emilio Ribas n.174, Campinas, SP, Brazil
Recommended Citation:
Silva P.H.M.D.,Sebbenn A.M.,Grattapaglia D.,et al. Realized pollen flow and wildling establishment from a genetically modified eucalypt field trial in Southeastern Brazil[J]. Forest Ecology and Management,2017-01-01,385