DOI: 10.1016/j.atmosenv.2015.02.075
Scopus记录号: 2-s2.0-84923923050
论文题名: Influence of elevated ozone concentration on methanotrophic bacterial communities in soil under field condition
作者: Huang Y ; Z ; , Zhong M
刊名: Atmospheric Environment
ISSN: 0168-2563
EISSN: 1573-515X
出版年: 2015
卷: 108 起始页码: 59
结束页码: 66
语种: 英语
英文关键词: Community
; Methanotrophic bacteria
; Ozone
; Real-time quantitative PCR
; Soil
; T-RLFP
; Winter wheat
Scopus关键词: Activated carbon
; Bacteria
; Climate change
; Genes
; Methane
; Ozone
; Polymerase chain reaction
; RNA
; Social sciences
; Soils
; Community
; Methanotrophic bacteria
; Real-time quantitative PCR
; T-RLFP
; Winter wheat
; Soil pollution
; ozone
; RNA 16S
; agricultural soil
; carbon cycle
; community structure
; concentration (composition)
; methane
; methanotrophy
; microbial community
; open-top chamber
; oxidation
; ozone
; soil microorganism
; wheat
; agricultural land
; analytic method
; Article
; carbon cycling
; concentration (parameters)
; controlled study
; gene dosage
; gene number
; methanotrophic bacterium
; microbial community
; nonhuman
; nucleotide sequence
; phylogenetic tree
; potential methane oxidation rate
; potential methane production rate
; priority journal
; quantitative analysis
; real time polymerase chain reaction
; restriction fragment length polymorphism
; soil depth
; soil fumigation
; soil microflora
; species composition
; Triticum aestivum
; winter wheat
; Bacteria (microorganisms)
; Triticum aestivum
; type II methanotrophic bacterium
Scopus学科分类: Environmental Science: Water Science and Technology
; Earth and Planetary Sciences: Earth-Surface Processes
; Environmental Science: Environmental Chemistry
英文摘要: The open top chamber (OTC) method was used in combination with real-time quantitative PCR and terminal restriction fragment length polymorphism (T-RFLP) techniques in the wheat field to study the influence of different levels of O3 concentrations (ambient air filtered by activated carbons, 40ppb, 80ppb and 120ppb) on the quantity and community structure of methanotrophic bacteria. O3 stress can influence the potential methane oxidation rate (PMOR) and potential methane production rate (PMPR) in the farmland soil. O3 treatment of 40ppb improved significantly the 16S rRNA gene copy number in the total methanotrophic bacteria pmoA, and type I and type II methanotrophic bacteria in the soil depth of 0-20cm. When the O3 concentration reached 120ppb, the 16S rRNA gene copy number in the total methanotrophic bacteria pmoA and type I methanotrophic bacteria decreased significantly as compared to the control treatment in 10-20cm layer. The 16s rRNA gene copy number of total methanotrophic bacteria pmoA and type I and type II methanotrophic bacteria were influenced by different O3 concentration and soil depth. The T-RFLP analysis indicated that O3 stress influenced significantly the community structure of the methanotrophic bacteria in soil, causing potential threat to the diversity of methanotrophic bacteria. It seems to imply that the rise of O3 concentration could produce an impact on the carbon cycling and the methane emission of the wheat field soil by changing the community structure and diversity of methanotrophic bacteria, which then influences the global climate change. © 2015 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/81842
Appears in Collections: 气候变化事实与影响
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作者单位: Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin, China; Department of Soil Sciences, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China
Recommended Citation:
Huang Y,Z,, Zhong M. Influence of elevated ozone concentration on methanotrophic bacterial communities in soil under field condition[J]. Atmospheric Environment,2015-01-01,108