| DOI: | 10.1016/j.atmosenv.2015.02.035
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| Scopus记录号: | 2-s2.0-84923568135
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| 论文题名: | Predominance of single bacterial cells in composting bioaerosols |
| 作者: | Galès A; , Bru-Adan V; , Godon J; -J; , Delabre K; , Catala P; , Ponthieux A; , Chevallier M; , Birot E; , Steyer J; -P; , Wéry N
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| 刊名: | Atmospheric Environment
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| ISSN: | 0168-2563
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| EISSN: | 1573-515X
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| 出版年: | 2015
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| 卷: | 107 | | 起始页码: | 225
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| 结束页码: | 232
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| 语种: | 英语
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| 英文关键词: | Aerodynamic diameter of bacteria
; Bioaerosol
; Compost
; Flow cytometry
; Particle-size distribution
; QPCR
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| Scopus关键词: | Aerodynamics
; Aerosols
; Aspergillus
; Composting
; Flow cytometry
; Light transmission
; Microorganisms
; Particle size
; Particle size analysis
; Plants (botany)
; Polymerase chain reaction
; Size distribution
; Aerodynamic diameters
; Aspergillus fumigatus
; Bioaerosols
; Electrical low-pressure impactor
; Geometric mean diameters
; QPCR
; Quantitative real time PCR
; Thermophilic microorganisms
; Bacteria
; aerodynamics
; aerosol
; atmospheric pollution
; bacterium
; compost
; composting
; flow cytometry
; fungus
; industrial emission
; particle size
; size distribution
; aerosol
; Article
; Aspergillus fumigatus
; bacterial cell
; composting
; flow cytometry
; nonhuman
; particle size
; priority journal
; real time polymerase chain reaction
; Saccharopolyspora rectivirgula
; Actinobacteria (class)
; Aspergillus fumigatus
; Bacteria (microorganisms)
; Fungi
; Saccharopolyspora rectivirgula
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| Scopus学科分类: | Environmental Science: Water Science and Technology
; Earth and Planetary Sciences: Earth-Surface Processes
; Environmental Science: Environmental Chemistry
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| 英文摘要: | Bioaerosols emitted from composting plants have become an issue because of their potential harmful impact on public or workers' health. Accurate knowledge of the particle-size distribution in bioaerosols emitted from open-air composting facilities during operational activity is a requirement for improved modeling of air dispersal. In order to investigate the aerodynamic diameter of bacteria in composting bioaerosols this study used an Electrical Low Pressure Impactor for sampling and quantitative real-time PCR for quantification. Quantitative PCR results show that the size of bacteria peaked between 0.95μm and 2.4μm and that the geometric mean diameter of the bacteria was 1.3μm. In addition, total microbial cells were counted by flow cytometry and revealed that these qPCR results corresponded to single whole bacteria. Finally, the enumeration of cultivable thermophilic microorganisms allowed us to set the upper size limit for fragments at an aerodynamic diameter of ~0.3μm. Particle-size distributions of microbial groups previously used to monitor composting bioaerosols were also investigated. In collected the bioaerosols, the aerodynamic diameter of the actinomycetes Saccharopolyspora rectivirgula-and-relatives and also of the fungus Aspergillus fumigatus, appeared to be consistent with a majority of individual cells. Together, this study provides the first culture-independent data on particle-size distribution of composting bioaerosols and reveals that airborne single bacteria were emitted predominantly from open-air composting facilities. © 2015 Published by Elsevier Ltd. |
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| 资源类型: | 期刊论文
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/81875
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| Appears in Collections: | 气候变化事实与影响
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作者单位: | INRA, UR0050 Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, Narbonne, France; Veolia Environnement Recherche et Innovation, 291 Avenue Dreyfous Ducas, Limay, France; CNRS, UMR 7621, Laboratoire d'Océanographie Microbienne, Observatoire Océanologique, Banyuls/mer, France; Sorbonne Universités, UPMC Univ Paris 06, UMR 7621, Laboratoire d'Océanographie Microbienne, Observatoire Océanologique, Banyuls/mer, France
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| Recommended Citation: | |
Galès A,, Bru-Adan V,, Godon J,et al. Predominance of single bacterial cells in composting bioaerosols[J]. Atmospheric Environment,2015-01-01,107
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